QI Ming-xing,ZHU Jie-hua,YANG Zhi-hui,et al.Phylogenetic Classification of Pectin Lyase Gene Family in the Genus Phytophthora[J].Northern Horticulture,2013,37(23):109-112.
疫霉属果胶裂解酶基因家族的系统发育分类
- Title:
- Phylogenetic Classification of Pectin Lyase Gene Family in the Genus Phytophthora
- 文章编号:
- 1001-0009(2013)23-0109-04
- Keywords:
- Phytophthora; pectin lyase; phylogenetic; specificit
- 分类号:
- S 432.1
- 文献标志码:
- A
- 摘要:
- 以疫霉属果胶裂解酶（pectin lyase，PL）基因家族的蛋白序列为材料，用邻接法和最小进化法构建系统发育树，根据所形成的分支确定PL基因家族的类群和分布规律。研究了疫霉属（Phytophthora）的果胶裂解酶基因家族的类群及在病原菌间的分布规律，以期为研究病原菌果胶裂解酶的专化性和病原菌的寄主专化性的关系奠定基础。结果表明：所构建的疫霉属PL基因家族的系统发育树共形成了11个分支，分别将其命名为PL1~PL11。其中，致病疫霉、橡树疫霉和大豆疫霉等3个病原疫霉属所共有的分支为5个，即PL1、PL6、PL7、PL10和PL11。橡树疫霉和大豆疫霉共有的分支为PL8。而上述3种疫霉均具其独有的1~2个分支，即PL4为橡树疫霉特有的分支，PL3和PL5为大豆疫霉的特有的分支，而PL2和PL9是致病疫霉特有的分支。利用疫霉果胶裂解酶基因家族的系统发育树的分支对其进行了分类和命名，并探究了疫霉属病原菌果胶裂解酶基因家族的分化与其寄主专化性的关系。
- Abstract:
- The protein sequences of pectin lyase gene family of Phytophthora were used to construct phylogenetic tree using neighbor-joining and minimum-evolution methods.According to the formed clades，classification and distribution of PL gene family were determined.The objective of this study was to investigate the classification of PL gene family of Phytophthora fungi and the gene distribution among different species of pathogens，which could laid the foundation of the relationship between the species specificity of pectin lyase genes and host specificity of pathogens.The results showed that the tree was divided into 11 clades，which were nominated as PL1~PL11，repectively.Of 11 clades，5 ones (i.e.PL1，PL6，PL7，PL10 and PL11) were shared by three Phytophthora species i.e.P.infestans,P.sojae and P.ramorum and PL8 was shared by P.sojae and P.ramorum.Nevertheless，three Phytophthora species had species-specific one or two clades.PL4 was specific to P.ramorum，PL3 and PL5 were specific to P.sojae and PL2 and PL9 were specific to P.infestans.The clades of phylogenetic tree were used to classify and nominate PL gene family of genus Phytophthora.The relationship of the division of PL gene family of Phytophthora pathogens and host specificity of pathogens was analyzed.
参考文献/References:
[1] Kroon L P，Brouwer H，de Cock A W，et al.The genus Phytophthora anno 2012[J].Phytopathology,2012,102(4):348-364.
[2] Haverkort A J，Boonekamp P M，Hutten R，et al.Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification[J].Potato Research,2008,51(1):47-57.
[3] 薛长湖,张永勤,李兆杰,等.果胶及果胶酶研究进展[J].食品与生物技术学报,2005,24(6):94-99.
[4] Pérez S，Mazeau K，Hervé du Penhoat C.The three-dimensional structures of the pectic polysaccharides[J].Plant Physiology and Biochemistry,2000,38(1):37-55.
[5] 杜继煜,白岚,白宝璋.果胶的化学组成与基本特性[J].农业与技术,2002,22(5):72-76.
[6] Reignault P，Valette-Collet O，Boccara M.The importance of fungal pectinolytic enzymes in plant invasion，host adaptability and symptom type[J].European Journal of Plant Pathology,2008,120(1):1-11.
[7] Vander Vlugt-Bergmans C，Meeuwsen P，Voragen A，et al.Endo-xylogalacturonan hydrolase，a novel pectinolytic enzyme[J].Applied and Environmental Microbiology,2000,66(1):36-41.
[8] 李祖明,张洪勋,白志辉,等.微生物果胶酶研究进展[J].生物技术通报,2010,33(3):42-48.
[9] Yadav S，Yadav P K，Yadav D，et al.Pectin lyase：a review[J].Process Biochemistry,2009,44(1):1-10.
[10]娜仁,赵君,张之为,等.马铃薯抗晚疫病机制的研究进展[J].中国马铃薯,2008,22(6):352-357.
[11]The Btosd institute[EB/OL].http://www.broadinstitute.org，2013-05-10/2013-05-20.
[12]Haas B J，Kamoun S，Zody M C，et al.Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans[J].Nature,2009,461(7262):393-398.
[13]Tyler B M，Tripathy S，Zhang X，et al.Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis[J].Science,2006,313(5791):1261-1266.
[14]Chenna R，Sugawara H，Koike T，et al.Multiple sequence alignment with the Clustal series of programs[J].Nucleic Acids Research,2003,31(13):3497-3500.
[15]Hall T A.BioEdit：a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[C].Nucleic Acids Symposium Seres,1999,41:95-98.
[16]chmidt H A，Strimmer K，Vingron M，et al.TREE-PUZZLE：maximum likelihood phylogenetic analysis using quartets and parallel computing[J].Bioinformatics,2002,18(3):502-504.
[17]Zhaxybayeva O，Gogarten J P.Bootstrap，Bayesian probability and maximum likelihood mapping：exploring new tools for comparative genome analyses[J].BMC Genomics,2002,3(1):4.
[18]Strimmer K，Von Haeseler A.Likelihood-mapping：a simple method to visualize phylogenetic content of a sequence alignment[J].Proceedings of the National Academy of Sciences,1997,94(13):6815-6819.
[19]Tamura K，Dudley J，Nei M，et al.MEGA4：molecular evolutionary genetics analysis (MEGA) software version 4.0[J].Molecular Biology and Evolution,2007,24(8):1596-1599.
[20]Rambaut A.FigTree version 131[EB/OL].http://tree.bio.ed.ac.uk/software/figtree，2011-02-11/2013-05-10.
[21]Zwickl D J，Hillis D M.Increased taxon sampling greatly reduces phylogenetic error[J].Systematic Biology,2002,51(4):588-598.
[22]Vincken J.Remodelling pectin structure in potato[J].Developments in Plant Genetics and Breeding,2000（6）:245-256.
[23]Frank S A.Models of plant-pathogen coevolution[J].Trends in Genetics,1992,8(6):213-219.
[24]Casadevall A，Pirofski L.Host-pathogen interactions：redefining the basic concepts of virulence and pathogenicity[J].Infection and Immunity,1999,67(8):3703-3713.
[25]MccannM C，Shi J，Roberts K，et al.Changes in pectin structure and localization during the growth of unadapted and NaCl-adapted tobacco cells[J].The Plant Journal,1994,5(6):773-785.
[26]Schols H A，Voragen A.Complex pectins：structure elucidation using enzymes[J].Progress in Biotechnology,1996（14）:3-19.
相似文献/References:
[1]李树江,孙爱群,杨友联.真菌感染性果树流胶病研究进展[J].北方园艺,2015,39(03):179.[doi:10.11937/bfyy.201503052]
LI Shu-jiang,SUN Ai-qun,YANG You-lian.Research Progress on Fungal Gummosis of Fruit Trees[J].Northern Horticulture,2015,39(23):179.[doi:10.11937/bfyy.201503052]
备注/Memo
第一作者简介：齐明星（1988-），男，河北保定人，硕士研究生，研究方向为植物病理学。E-mail:qi_mingxing@hotmail.com.
责任作者：杨志辉（1975-），男，河北藁城人，博士，副教授，硕士生导师，研究方向为植物病理学。E-mail:bdyangzhihui@163.com.
基金项目：国家自然科学基金青年基金资助项目（31000833）；国家公益性行业（农业）科研专项资助项目（201303018）。
收稿日期：2013-06-19