SHI Yu,JIANG Shi-cui,ZHANG Mei-ping,et al.Research Progress About Transformation of Large DNA Fragments[J].Northern Horticulture,2013,37(15):208-211.
大片段DNA遗传转化的研究进展
- Title:
- Research Progress About Transformation of Large DNA Fragments
- 文章编号:
- 1001-0009(2013)15-0208-04
- Keywords:
- cloning vector; large fragments; genetic transformation
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- DNA克隆技术是分子生物学研究中一项重要的技术手段。随着分子生物学技术的发展及植物基因组计划的进行,产生了既能够克隆大片段DNA又能够将候选基因利用农杆菌介导法进行遗传转化试验的载体。现对大片段克隆载体的发展及大片段DNA遗传转化的影响因素进行综述，并对大片段DNA遗传转化的发展作了展望。
- Abstract:
- DNA cloning technology is an important means of technology in molecular biology research.With the development of molecular biology techniques and plant genome project progresses,the vector both capable of cloning large DNA fragments and candidate genes using Agrobacteriummediated genetic transformation experiments were produced.The development of large fragment cloning vector,and influencing factors of genetic transformation of large DNA fragments were summarized,and the development of genetic transformation of large DNA fragments were prospected in this paper.
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备注/Memo
第一作者简介：石玉（1987-），女，硕士研究生，研究方向为植物分子生物学与基因工程。E-mail：shiyu0608@gmail.com.
责任作者：王义（1966-），男，博士，教授，研究方向为药用植物细胞工程。E-mail:wanglaoshi2007@tom.com.
收稿日期：2013-03-27