[1]周书利,汤浩茹,代利娟,等.不同因素对草莓试管苗玻璃化的影响[J].北方园艺,2013,37(13):131-134.
　ZHOU Shu-li,TANG Hao-ru,DAI Li-juan,et al.Effects of Different Factors on Vitrification of Strawberry Shoots[J].Northern Horticulture,2013,37(13):131-134.

点击复制

不同因素对草莓试管苗玻璃化的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷期数: 2013年13期页码: 131-134 栏目: 出版日期: 2013-07-15

Title:: Effects of Different Factors on Vitrification of Strawberry Shoots

文章编号:: 1001-0009（2013）13-0131-04

作者:: 周书利¹; 汤浩茹¹; 2; 代利娟¹; 罗霞¹; 1.四川农业大学园艺学院，四川雅安 625014；
2.四川农业大学果蔬研究所，四川成都 611130

Author(s):: ZHOU Shu-li¹; TANG Hao-ru¹; 2; DAI Li-juan¹; LUO Xia¹; 1.College of Horticulture，Sichuan Agricultural University，Ya’an，Sichuan 625014;
2.Institute of Pomology and Olericulture,Sichuan Agricultural University，Chengdu,Sichuan 611130

关键词:: 草莓试管苗; 正交设计; 玻璃化; 生理生化

Keywords:: strawberry shoot; orthogonal design; vitrification; physiology and biochemistry

分类号:: S 668-4

文献标志码:: A

摘要:: 以“丰香”草莓丛生芽为外植体，采用正交实验设计，研究了6BA、琼脂、铵离子不同浓度组合对草莓试管苗玻璃化的影响，同时测定了玻璃化试管苗与正常苗的生理指标差异。结果表明：3种因素对草莓试管苗玻璃化均有显著影响，影响大小依次为6BA >铵离子>琼脂；能有效降低试管苗玻璃化的组合为1.0 mg/L 6BA+6.0 g/L琼脂+1 650 mg/L铵离子，增殖倍数也较高；玻璃化苗的组织含水量和膜透性较正常苗高，可溶性糖含量则相反；玻璃化苗的叶绿素含量较正常苗显著降低，叶绿素a/叶绿素b在正常苗与玻璃苗间无显著差异，玻璃化苗较正常苗超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性降低，过氧化物酶（POD）活性升高。

Abstract:: Taking ‘Toyonoka’ strawberry bud as explants,by method of orthogonal design，different combinations of 6BA，agar，ammonium ion (ammonium chloride) were added to subculture medium of strawberry，the effects on vitrification of strawberry shoots were studied.Meanwhile，physiological and biochemical indexes between vitrification shoots and normal plantlets were determined and compared.The results showed that there were significant differences in the vitrification rate of shoots among 9 treatments.Analysis of variance showed that 6BA was the most significant impactor on strawberry vitrification，the second was ammonium ion，and agar affected least.The optimum one was 1.0 g/L 6BA+6.0 g/L agar+1 650 mg/L ammonium ion and the vitrification rate only was 26.22%,with higher proliferation ratio.Organization moisture content and membrane permeability of vitrification shoots were higher than normal plantlets，soluble sugar on the contrary.Chlorophyll content of vitrification shoots was significantly lower than normal plantlets，but there had no significant differences of Chla/Chlb between normal plantlets and vitrification shoots.Oxide dismutase (SOD) and catalase (CAT) activity of vitrification shoots were reduced compared with normal plantlets，while peroxidase (POD) activity increased.

参考文献/References:

[1]万清林,赵书清.草莓果实营养成分的分析[J].北方园艺,1994(6):34-35.

?[2]郭月玲,解振强,王永平.我国草莓组织培养生产研究现状及前景[J].浙江农业科学,2010(6):1211-1215.

[3]李胜,李唯,杨德龙,等.植物试管苗玻璃化现象研究进展[J].甘肃农业大学学报,2002,3(1):1-6.

[4]杨俊英,罗庆熙,宋明,等.大蒜试管苗玻璃化机理的研究[J].西南农业学报,2005,18(6):801-805.

[5]张敏,张曙光.对影响草莓组培苗玻璃化若干因素的探讨[J].湖北农业科学,2002(6):82-83.

[6]曹善东.组培条件对草莓脱毒试管苗玻璃化影响的研究[J].山东农业大学学报（自然科学版）,2006,37(2):172-174.

[7]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:19-20.

[8]许大全.叶绿素含量的测定及其应用中的几个问题[J].植物生理学通讯,2009,45(9):896-898.

[9]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:195-197.

[10]陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2006:64-66.

[11]邹琦.植物生理学实验指导[M].北京:中国农业出版社，2000:163-165,168-169.

[12]陈兵先,黄宝灵,吕成群,等.植物组织培养试管苗玻璃化现象研究进展[J].林业科技开发,2011,25(1):1-5.

[13]李海刚,孔祥生.牡丹试管苗玻璃化的研究[J].生物学杂志,2010,27(5):35-42.

[14]赵佐敏.非洲菊组培苗玻璃化控制研究初报[J].贵州农业科学,2005,33(3):77.

[15]李佳莹,俞明亮,马瑞娟,等.桃组织培养中影响玻璃化产生的因素分析[J].江苏农业科学,2009(5):47-49.

[16]曹天旭,朴炫春,廉美兰,等.满天星试管苗玻璃化苗与正常苗的比较研究[J].黑龙江农业科学,2009(5):86-88.

[17]孙庆春,郑成淑,丰震.菊花玻璃化苗与正常苗的生理特征比较[J].山东农业科学,2009(5):45-47.

[18]周菊花,林证明,梁海曼.控制瑞香试管苗玻璃化的研究[J].园艺学报,1990,17(3):229-232.

[19]杨芸,吴震,李翠花,等.外源H₂O₂胁迫对大蒜试管苗玻璃化的影响[J].西北植物学报,2007,27(8):1637-1642.

[20]客绍英,张胜珍,刘玉军.菘蓝试管苗玻璃化过程中抗氧化物酶活性的变化[J].华北农学报,2009,24(3):154-158.

[21]王继芳,李锡香,贾春兰.丝石竹玻璃苗生理特性和形成机理初探[J].农业生物技术学报,1997(1):72-78.

相似文献/References:

[1]李光,余霜.应用正交设计优化甘薯叶绿原酸提取工艺研究[J].北方园艺,2014,38(11):119.
　LI Guang,YU Shuang.Study on Extraction Technology for Chlorogenic Acid From Sweet Potato Leaves by Orthogonal Design[J].Northern Horticulture,2014,38(13):119.
[2]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
　JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(13):108.
[3]任吉君,王艳,肖华凤.香薷总黄酮萃取工艺参数优化研究[J].北方园艺,2013,37(14):140.
　REN Ji-jun,WANG Yan,XIAO Hua-feng.Study on Optimized Extraction of Total Flavones from Elsholtzia ciliata Thunb.[J].Northern Horticulture,2013,37(13):140.
[4]郑平生.外源激素对盐胁迫下草莓试管苗酶活性的影响[J].北方园艺,2013,37(06):116.
　ZHENG Ping-sheng.Study on Exogenous Hormones on the Activity of Membrane Protective Enzymes of Strawberry Plantlets in Salt Stress[J].Northern Horticulture,2013,37(13):116.
[5]王京,张晓明,乔枫,等.玉树地区独一味ISSR-PCR反应体系的建立与优化[J].北方园艺,2013,37(09):126.
　WANG Jing,ZHANG Xiao-ming,QIAO Feng,et al.Establishment and Optimization of ISSR-PCR Reaction System for Yushu Lamiophlomis rotata (Benth.) Kudo[J].Northern Horticulture,2013,37(13):126.
[6]郝凤,于铁峰,雷银川.番茄SRAP-PCR反应体系建立与优化[J].北方园艺,2012,36(03):107.
　HAO Feng,YU Tie-feng,LEI Yin-chuan.Optimization for SRAP-PCR System of Tomato Based on Orthogonal Design[J].Northern Horticulture,2012,36(13):107.
[7]吴春燕,高义,宋廷宇,等.正交设计优化大白菜ISSR-PCR 反应体系[J].北方园艺,2012,36(05):127.
　WU Chun-yan,GAO Yi,SONG Ting-yu,et al.Optimization of an ISSR-PCR Reaction System of Chinese Cabbage by Orthogonal Design[J].Northern Horticulture,2012,36(13):127.
[8]邓俭英,万正林,李立志,等.不同施肥处理对黑皮冬瓜产量的影响[J].北方园艺,2012,36(16):131.
　DENG Jian-ying,WAN Zheng-lin,LI Li-zhi,et al.Effects of Different Fertilizer Treatments on Yield of Black Wax Guard[J].Northern Horticulture,2012,36(13):131.
[9]李亚慧,黄丛林,董然.菊花SSR-PCR反应体系的建立和优化[J].北方园艺,2012,36(13):127.
　LI Ya-hui,HUANG Cong-lin,DONG Ran.Establishment and Optimization of Chrysanthemum SSR-PCR Reaction System[J].Northern Horticulture,2012,36(13):127.
[10]李颖,谢占玲,田飞,等.黄绿蜜环菌SSR-PCR反应体系的优化及引物筛选[J].北方园艺,2013,37(16):114.
　LIYing,XIEZhan-ling,TIANFei,et al.EstablishmentandPrimerScreeningoftheSSR-PCRReactionSystemfor Armillarialuteo-virens[J].Northern Horticulture,2013,37(13):114.

备注/Memo

第一作者简介：周书利（1988-），女，硕士，研究方向为植物生物技术在果树上的应用。E-mail：0616101099@163.com.

责任作者：汤浩茹（1963-），男，博士，教授，博士生导师，现主要从事植物生物技术在果树上的应用与果树种子资源及遗传育种研究工作。E-mail：htang@sicau.edu.cn.

收稿日期：2013-03-07

更新日期/Last Update: 2014-08-08