BAO Lin-wen,CHEN Zhe-hao,ZHONG Dan,et al.Effects of Different Hormones on Buds Regeneration Induction from ?Cotyledons in Cucumber ‘Ludi No.2’[J].Northern Horticulture,2013,37(13):100-103.
不同激素组合对黄瓜“露地二号”离体子叶再生芽诱导的影响
- Title:
- Effects of Different Hormones on Buds Regeneration Induction from ?Cotyledons in Cucumber ‘Ludi No.2’
- 文章编号:
- 1001-0009(2013)13-0100-04
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以黄瓜品种“露地二号”为试材,以MS为基本培养基,以3d苗龄的子叶为外植体,研究比较了不同植物激素组合以及AgNO3对离体子叶再生芽诱导的影响。结果表明:在6BA 2.0 mg/L+ABA 1.0 mg/L+AgNO3 2.0 mg/L的组合下,黄瓜离体子叶再生芽的分化率最高可达76.67%,该项研究为优化黄瓜离体子叶再生芽诱导体系及进一步建立快速、稳定、高效的黄瓜离体植株再生体系,开展黄瓜转基因工作奠定了基础。
参考文献/References:
[1]薛丹丹,张凤生,王保菊,等.黄瓜再生体系的建立[J].北方园艺,2010(7):119-121.
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备注/Memo
第一作者简介:鲍林文(1987-),女,硕士研究生,现主要从事植物发育与基因工程等研究工作。E-mail:jessirybao@163.com.