FANG Hua-zhou.Study on the Relationship Between Cultural Characteristics and Results of Weeds of Cordyceps militaris[J].Northern Horticulture,2013,37(12):161-164.
蛹虫草菌种培养性状与出草结果关系的研究
- Title:
- Study on the Relationship Between Cultural Characteristics and Results of Weeds of Cordyceps militaris
- 文章编号:
- 1001-0009（2013）12-0161-04
- 分类号:
- S 646.9
- 文献标志码:
- A
- 摘要:
- 以不同保藏期及传代次数菌种为试验组，以优质母株为对照组，研究比较了各菌种培养性状与出草结果之间的关系，以期为鉴别优质蛹虫草菌种提供依据。结果表明：菌种保藏1月或传代1次，固体平板培养菌丝洁白健壮、气生菌丝少匍匐状、长势好、转色快、转色深，液体培养菌丝球细密、粘稠、生物量高，出草形态好、产量最高；菌种保藏超过6月或传代超过3次，则菌丝色泽变暗、气生菌丝多呈棉絮状、转色迟、转色淡，液体培养菌丝球数量少、粗大、生物量低，产量显著较低。
- Abstract:
- Taking the bacterial strains come from different storage periods and the times of passage as experimental group，regarding quality material as matched group，the relationship between cultural characteristics and results of weeds were studied,in order to provide a basis for identification of high quality strains of Cordyceps militaris.The results showed that if the strains were stored for a month or continuous cell culture only for one time，bacterial strains by solid culture were white and strong，little creeping shaped hyphae，growth better，turned color faster and turned color deeper，and bacterial strains by liquid culture had close，thick，high-biomass bacterial strains and the most production;while if stored for more than six months and continuous cell culture more than three times，bacterial strains by liquid culture were gloomy，much gossypine，turned color later and weaker，and bacterial strains by liquid culture were thick，little production，low-biomass and the production obviously got low.
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备注/Memo
作者简介：方华舟(1965-)，男，湖北黄冈人，副教授，研究方向为食用菌技术与农业微生物学。E-mail：fanghuazhou2000@yahoo.com.cn.
基金项目：湖北省教育厅重点科研资助项目(D20126101)。
收稿日期：2012-03-13