[1]徐玲玲,党悦方,陶贵荣,等.月季“圣斯威辛”的快速繁殖技术研究[J].北方园艺,2013,37(12):97-99.
　XU Ling-ling,DANG Yue-fang,TAO Gui-rong,et al.Study on the Rapid Propagation of Rosa ‘St.Stwithum’[J].Northern Horticulture,2013,37(12):97-99.
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月季“圣斯威辛”的快速繁殖技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年12期 页码: 97-99 栏目: 出版日期: 2013-06-15
Title:
Study on the Rapid Propagation of Rosa ‘St.Stwithum’
文章编号:
1001-0009(2013)12-0097-03
作者:
徐玲玲1; 党悦方1; 陶贵荣1; 裴昕2; 郭斌3
1.西安文理学院 生物技术学院，陕西 西安 710065；
2.西安芳田玫瑰农业科技有限公司，陕西 西安 710061；
3.西北大学 生命科学学院，西部资源生物与现代生物技术教育部重点实验室，陕西 西安 710069
Author(s):
XU Ling-ling1; DANG Yue-fang1; TAO Gui-rong1; PEI Xin2; GUO Bin3
1.School of Biotechnology，Xi’an University of Arts and Science，Xi’an，Shaanxi 710065;
2.Xi’an Fangtian Rose Agricultural Science and Technology Co.，Ltd.，Xi’an，Shaanxi 710061;
3.Key Laboratory of Resource Biology and Biotechnology in Western China，School of Life Science，Northwest University，Xi’an，Shaanxi 710069
关键词:
“圣斯威辛”月季; 组织培养; 不定芽; NAA; IBA
Keywords:
Rosa ‘ St.Stwithum’; tissue culture; adventitious bud; NAA; IBA
分类号:
S 685.12
文献标志码:
A
摘要:
以“圣斯威辛”（Rosa ‘St.Stwithum’）月季带腋芽的节为外植体,研究了“圣斯威辛”月季不定芽试管生根的最佳条件。结果表明：0.05 mg/L NAA和3 mg/L 6-BA的MS培养基中月季不定芽的诱导频率和平均每个外植体上的芽数最多，分别为95.7%和8.4个。将生长至3 cm的不定芽转移到生根培养基上诱导根的形成，无激素处理的月季组培苗的平均每个不定芽生根数为6.3条，根诱导频率92%，平均根长为3.7 cm。该结果与添加低浓度的IBA（0.2 mg/L）对根的诱导效果没有显著性差异。高浓度的NAA和IBA诱导出大量的愈伤组织，不定根的数量显著减少。将生根的月季试管苗移栽到温室中，移栽成活率达到92%。
Abstract:
Taking the axillary buds of Rosa ‘St.Stwithum’ as explants，an efficient micropropagation system for Rosa ‘St.Stwithum’ were studied.The results showed that 95.7% adventitious buds induction frequency and 8.4 buds per explant were achieved when cultured on MS media containing 0.05 mg/L NAA and 3 mg/L 6-BA.After transferred the adventitious buds，which length had grown to 3 cm，to rooting media，root organogenesis occurred.The control without any exogenous auxins treatment，which average roots number per stem，induction frequency and the average roots’ length were 6.3 roots，92% and 3.7 cm，respectively，had no significant differences with 0.2 mg/L IBA treatments.High concentrations of NAA and IBA treatments induced a lot of callus，and significantly decreased adventitious roots.92% of the regenerated plantlets survived and grew vigorously in greenhouse condition.

参考文献/References:

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备注/Memo

第一作者简介：徐玲玲（1981-），女，山东烟台人，博士，讲师，现主要从事植物与微生物的教学与科研工作。E-mail:missilexll@163.com.
责任作者：郭斌（1975-），男，陕西华县人，博士，讲师，现主要从事植物生理学的教学与科研工作。E-mail:guobin@nwu.edu.cn.
基金项目：国家自然科学基金资助项目（31000144）；陕西省自然科学基础研究计划资助项目（2012JQ3017）；西安市科技计划资助项目（NC1206(3)）；陕西省教育厅基金资助项目（12JK0846）。
收稿日期：2013-01-15

更新日期/Last Update: 2014-08-19