JIANG Xing-jian,WANG Chun-hui,PENG Yun-xiang,et al.Detection of the Laccase and MCMase Activities of Agrocybe cylindracea During Its Growth[J].Northern Horticulture,2013,37(11):149-151.
白色茶树菇生长发育中漆酶与纤维素酶活力的测定
- Title:
- Detection of the Laccase and MCMase Activities of Agrocybe cylindracea During Its Growth
- 文章编号:
- 1001-0009(2013)11-0149-03
- Keywords:
- Agrocybe cylindracea; laccase; CMCase; enzyme activity
- 分类号:
- S 646.1+9
- 文献标志码:
- A
- 摘要:
- 以“湘茶158”白色茶树菇为试材，分别采用ABTS法和DNS法对其进行了液体发酵和出菇培养2个阶段的漆酶与羧甲基纤维素酶活性的测定，以探讨菌丝体对培养基质中纤维素的分解能力。结果表明：液体发酵培养阶段，前6 d漆酶活性较低，6 d之后迅速上升，第8天达到最高峰值，之后酶活性峰值又急剧下降，最后趋于缓和；CMC酶活性前4 d较低，之后迅速上升，第6天达到最高峰值，之后酶活性又逐渐下降，最后趋于缓和。出菇培养阶段，菌丝长满半袋和满袋时，漆酶活性处于很低的水平，1潮菇原基时，酶活峰值急速升至较高水平，幼菇时达到最高峰值，之后又急剧下降，在2潮菇的几个阶段，漆酶活性均处于较低水平；CMC酶活性在菌丝长满半袋和满袋时处于一般水平，而在1潮菇原基时期，酶活峰值急速升至最高峰值，在幼菇时期又急剧下降至一般水平，在1潮菇子实体成熟期和二潮菇的几个阶段，CMC酶活性变化不大，均处于较低水平。
- Abstract:
- Aim to analysis the degradation ability of mycelia to cellulose，laccase and CMCase activitites from Agrocybe cylindracea were tested in the liquid and fruiting body cultured periods by using ABTS method and DNS method，respectively.The results showed that in the liquid period the laccase activity and CMCase activity were in a low level in the first 6 and 4 days.Then the activities were hastily rised，it was highest up to the 8th and the 6th days and then it was hastily declined until to a low and relaxative level，respectively.In the fruiting body cultured period，the laccase activity and CMCase activity were in a low and common level when the mycelia grow to a half cultural bag，respectively.The laccase activity was a higher level in the first time of primordium formation，and the highest level in the first time of young fruiting body formation.However，the CMCase activity was in a common level before the mycelia grow full of the cultural bag，then up to the highest level in the first time of primordium formation，and then hastily declined to a common level.The laccase activity and CMCase activity were both in a low level in the second fruiting body period.This study provided a reference for the analysis of physiological activity and culture production and utilization of A.cylindracea.
参考文献/References:
[1]路等学,王龙,张铎,等.茶薪菇胞外酶学性质研究[J].中国酿造,2010(2):37-39.
[2]才晓玲,安福全,邹瑶,等.柱状田头菇研究进展[J].食用菌学报,2011,18(2):65-69.
[3]Uhart M,Piscera J M,Albertó E.Utilization of new naturally occurring strains and supplementation to improve the biological efficiency of the edible mushroom Agrocybe cylindrace a[J].Ournal of Industrial Microbiology and Biotechnology,2008,35:595-602.
[4]赵爽,刘宇,许峰,等.姬松茸4个品种菌丝体的生物酶活性测定[J].中国食用菌,2010,29(3):32-33.
[5]姜性坚,王春晖,彭运祥,等.5个姬菇菌株不同生长阶段漆酶及纤维素酶活力测定[J].食用菌,2010(6):9-10. [6]黄年来,林志彬,陈国良.中国食药用菌学[M].上海:上海科学技术文献出版社,2010:607-619.
[7]初洋,倪新江,杨佳文,等.姬菇和鲍鱼菇生长期间8种胞外酶活性变化比较[J].烟台大学学报(自然科学与工程版)，2008,21(2):138-142.
[8]郭倩,何庆邦.四孢蘑菇生长过程中四种胞外酶活性和木质纤维素降解的变化规律[J].食用菌学报,1998,5(2):13-17.
[9]Claydon N,Allar M,Wood D A.Fruitbody biomass regulated production of extracellular endocellulase during periodic fruiting by Agaricus bisporus[J].Trans Br Mycol Sci,1988,90(1)：85-90.
[10]暴增海,马桂珍,吴智艳.鸡腿蘑对基质的降解及有关酶活性变化特点研究[J].北方园艺,2002(3):54-55.
相似文献/References:
[1]何莹莹,陈卫民,赵永昌,等.云南田头菇属两个物种遗传多样性的AFLP分析[J].北方园艺,2012,36(21):85.
HE Ying-ying,CHEN Wei-min,ZHAO Yong-chang,et al.Genetic Diversity Analysis of Two Agrocybe Species in Yunnan by AFLP[J].Northern Horticulture,2012,36(11):85.
[2]杨红澎,班立桐,黄亮,等.新疆和天津产茶树菇中氨基酸和多糖的对比分析[J].北方园艺,2014,38(16):137.
YANG Hong-peng,BAN Li-tong,HUANG Liang,et al.Comparison and Analysis of Amino Acid and Polysaccharides in Agrocybe Cylindracea from Xinjiang and Tianjin[J].Northern Horticulture,2014,38(11):137.
[3]彭滟钞,曹福祥,董旭杰,等.漆酶的发酵生产及其应用研究进展[J].北方园艺,2013,37(24):206.
PENG Yan-chao,CAO Fu-xiang,DONG Xu-jie,et al.Research Progress on Fermentation Production and Application of Laccase[J].Northern Horticulture,2013,37(11):206.
[4]吴周斌,张健,王佳敏,等.不同培养料对真姬菇胞外酶活性的影响[J].北方园艺,2015,39(08):145.[doi:10.11937/bfyy.201508038]
WU Zhou-bin,ZHANG Jian,WANG Jia-min,et al.Effect of Different Cultivation Substrates on Extracellular Enzyme Activities of Hypsizygus marmoreus[J].Northern Horticulture,2015,39(11):145.[doi:10.11937/bfyy.201508038]
[5]艾 良,朱 启 忠,李 文 静,等.白腐真菌 F9 产漆酶发酵条件的优化[J].北方园艺,2010,34(04):0.[doi:10.11937/bfyy.201004053]
AI Liang,ZHU Qi- zhong,LI Wen - jing,et al.Studies on Fermentation Conditions for Laccase Production from White- Rot Fungi F9[J].Northern Horticulture,2010,34(11):0.[doi:10.11937/bfyy.201004053]
[6]黄万兵,桂阳,龚光禄,等.七株贵州蜜环菌胞内多糖得率及胞外酶活性研究[J].北方园艺,2016,40(02):134.[doi:10.11937/bfyy.201602035]
HUANG Wanbing,GUI Yang,GONG Guanglu,et al.Research on Intracellular Polysaccharide Yield and Extracellular Enzyme Activity Change of Armillarilla Strains Isolated From Guizhou[J].Northern Horticulture,2016,40(11):134.[doi:10.11937/bfyy.201602035]
[7]刘敏,卢红,黄媛媛,等.茶树菇液体发酵条件研究[J].北方园艺,2016,40(11):142.[doi:10.11937/bfyy.201611037]
LIU Min,LU Hong,HUANG Yuanyuan,et al.Study on the Liquid Fermentation Condition of Agrocybe aegerita(Brig.) Sing[J].Northern Horticulture,2016,40(11):142.[doi:10.11937/bfyy.201611037]
[8]孙荟林,李明,李守勉,等.茶树菇母种培养基最适碳氮比及碳源、氮源筛选[J].北方园艺,2016,40(14):148.[doi:10.11937/bfyy.201614037]
SUN Huilin,LI Ming,LI Shoumian,et al.Screening of Optimal Carbon-Nitrogen Ratio，Carbon Source Material and Nitrogen Source Material of the Mother Stage of Agrocybe aegerita[J].Northern Horticulture,2016,40(11):148.[doi:10.11937/bfyy.201614037]
[9]高小朋,贺晓龙,任桂梅,等.茶树菇组织分离母种及培养基的筛选[J].北方园艺,2011,35(10):0.[doi:10.11937/bfyy.2011010046]
GAO Xiao-peng,HE Xiao-long,REN Gui-mei,et al.The Tissue Isolation and Selection Stock Culture Medium of Agrocybe cylindracea[J].Northern Horticulture,2011,35(11):0.[doi:10.11937/bfyy.2011010046]
[10]舒 丽,陈 强,陈 翠 萍,等.野生茶树菇 YW- 2生物学特性及驯化栽培[J].北方园艺,2007,31(08):0.[doi:10.11937/bfyy.200708090]
[J].Northern Horticulture,2007,31(11):0.[doi:10.11937/bfyy.200708090]
备注/Memo
第一作者简介：姜性坚（1964-），男，本科，副研究员，现主要从事食用菌育种与栽培等研究工作。
基金项目：湖南省自然科学基金资助项目（12JJ3022）。
收稿日期：2013-01-16