WANG Na,GUO Tai-jun,LI Xue,et al.Study on Tissue Culture and Rapid Propagation of Lilium dauricum and Lilium cernum[J].Northern Horticulture,2013,37(11):91-94.
野生毛百合与松叶百合组培快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation of Lilium dauricum and Lilium cernum
- 文章编号:
- 1001-0009(2013)11-0091-04
- Keywords:
- Lilium dauricum; Lilium cernum; tissue culture
- 分类号:
- S 682.2+65
- 文献标志码:
- A
- 摘要:
- 以毛百合与松叶百合不同部位的鳞片为外植体，研究了不同消毒方式、不同激素组合和不同部位鳞片对毛百合和松叶百合不定芽诱导和增殖的影响。结果表明：毛百合用75%酒精30 s+30% 84消毒液15 min消毒效果最好；鳞茎的最佳诱导培养基为MS+0.5 mg/L 6-BA+0.5 mg/L NAA；最佳不定芽增殖培养基MS+0.3 mg/L 6-BA+0.2 mg/L NAA。松叶百合鳞茎的最佳诱导培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA；芽的增殖培养基为MS+1.0 mg/L 6-BA+0.1 mg/L 2,4-D。外植体取样部位以鳞茎中层鳞片的基部较好。
- Abstract:
- Taking different parts of squama of the bulb scales of Lilium dauricum and L.cernum as explants,the effect of different sterilize method and different hormones composing and different parts of a squama on their adventitious bud induction and multiplication were studied.The results showed the bulb scales treated with 75% alcohol for 30 s followed by 30% 84 disinfectant for 15 min exhibited the best sterilization effect；the suitable medium for bulb induction of L.dauricum was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L；the optimum medium for adventitious bud proliferation was MS+6-BA 0.3 mg/L+NAA 0.2 mg/L.The suitable medium for bulb induction of L.cernum was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L was better for adventitious bud proliferation.The better sampling sites of explants were the base of the bulbs central scales.
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备注/Memo
第一作者简介：汪娜（1987-），女，硕士研究生，研究方向为园林植物种质资源。E-mail:wangnawangna19870205@126.com.
责任作者：郭太君（1957-），男，硕士，教授，现主要从事园林种质资源与栽培生理生态等研究工作。E-mail:guoguo5557@126.com.
基金项目：农业部“吉林省农业野生植物调查”资助项目;吉林市科技局资助项目。
收稿日期：2013-01-16