[1]刘威,蓝祖栽,叶云峰,等.药水苏白绢病病原鉴定及其生防木霉菌株筛选[J].北方园艺,2013,37(05):125-128.
LIU Wei,LAN Zu-zai,YE Yun-feng,et al.Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain[J].Northern Horticulture,2013,37(05):125-128.
点击复制
LIU Wei,LAN Zu-zai,YE Yun-feng,et al.Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain[J].Northern Horticulture,2013,37(05):125-128.
药水苏白绢病病原鉴定及其生防木霉菌株筛选
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第37卷
期数:
2013年05期
页码:
125-128
栏目:
出版日期:
2013-03-15
- Title:
- Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain
- 文章编号:
- 1001-0009(2013)05-0125-04
- Keywords:
- Betonica officinalis; Sclerotium rolfsii; identification of the pathogen; antagonistic trichoderma strain; screen
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以药水苏白绢病株为试材,通过致病性测定法确定病原菌,采用病原菌的形态鉴定方法并结合该菌的ITS rDNA序列分析鉴定病原菌种类;采用平皿对峙法筛选拮抗菌株。结果表明:药水苏白绢病的病原菌为罗氏白绢小菌核菌(Sclerotium rolfsii Sacc.);5个供试木霉菌株中以哈茨木霉(H2)对病原菌的抑制作用最强,拮抗指数为Ⅱ级,抑菌率为86.27%。
- Abstract:
- Taking diseased plants of Betonica officinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.
参考文献/References:
[1]冉先德.中华药海(下册)[M].哈尔滨:哈尔滨出版社,1993:1297.
[2]方中达.植病研究方法[M].北京:中国农业出版社,1998:194.
[3]魏景超.真菌鉴定手册[M].上海:上海科技出版社,1979:645-648.
[4]易润华,朱西儒,周而勋.简化CTAB法快速微量提取丝状真菌DNA[J].湛江海洋大学学报,2003,23(6):72-73. [5]曹玉桃,姚革,文成敬,等.木霉拮抗黄瓜枯萎病菌菌株的筛选[J].西南农业学报,2007,20(3):408-411.
[6]张丽荣,康萍芝,沈瑞清.土壤菌对土传病害病原真菌的拮抗作用[J].内蒙古农业科技,2007(5):48-50.
[7]金苹,高晓余.白绢病的研究[J].农业灾害研究,2011(1):14-22.
[8]戴芳澜.中国真菌总汇[M].北京:科学出版社,1979:1060.
备注/Memo
第一作者简介:刘威(1982-),女,广西北流人,本科,助理工程师,现主要从事药用植物病虫害等研究工作。
基金项目:广西省卫生厅自筹基金资助项目(Z2011161)。
收稿日期:2012-11-09
更新日期/Last Update:
2014-08-22