LV Zhao,ZHANG Li-ping,CHENG Hui-cai,et al.Optimization of Fermentation for Production Conditions of Antibiotic by Bacillus amyloliquefaciens D1[J].Northern Horticulture,2013,37(05):94-97.
响应面法优化解淀粉芽孢杆菌D1产抗菌素培养基研究
- Title:
- Optimization of Fermentation for Production Conditions of Antibiotic by Bacillus amyloliquefaciens D1
- 文章编号:
- 1001-0009(2013)05-0094-04
- 分类号:
- S 482.2+8
- 文献标志码:
- A
- 摘要:
- 以1株解淀粉芽孢杆菌为出发菌，采用Box-Behnken试验及响应面分析相结合的方法对其产抗菌素的发酵培养基进行优化。结果表明：最佳培养基配方为C源淀粉1.88%，N源牛肉膏1.76%，酵母膏0.74%，KH2PO4 0.25%，K2HPO4 0.1%，(NH4)2SO4 0.08%。通过优化产抗菌素培养基，抗菌素的抑菌圈直径由优化前的19.2 mm提高到优化后的27.2 mm，提高了41.7%。
- Abstract:
- The Box-Behnken experiment and response surface analysis method were combined to optimize the fermentation medium of a Bacillus amyloliquefaciens.The results showed that the optimal fermentation medium formla consisted of 1.88% starch，1.76% beef extract，0.74% yeast extract，KH2PO2 0.25%，K2HPO20.1%，(NH4)2SO4 0.08%.There was a 41.7% increase in the yield of antibacterial peptides obtained using the optimal fermentation medium，compared with that before optimization.
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备注/Memo
第一作者简介：吕钊（1987-），男，硕士，研究方向为应用微生物学。E-mail:lv_zhao@hotmail.com.
责任作者：张丽萍（1969-），女，硕士，研究员，研究方向为应用微生物学。E-mail:lizzle-69@163.com.基金项目：国家农业成果转化基金资助项目（2010GB2A200041）；河北省科技支撑计划资助项目（10205520D）；河北省科学院重大攻关计划资助项目（12306）。
收稿日期：2012-11-08