YU Ya,QU Ling.Establishment of Highly Efficient in vitro Plant Regeneration of Pyrethrum cinerariaefolium[J].Northern Horticulture,2012,36(24):135-139.
除虫菊高效再生体系的建立
- Title:
- Establishment of Highly Efficient in vitro Plant Regeneration of Pyrethrum cinerariaefolium
- 文章编号:
- 1001-0009（2012）24-0135-05
- Keywords:
- Pyrethrum cinerariaefolium; adventitious buds; browning
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以除虫菊为试材，以带柄叶片为外植体，对除虫菊不定芽的诱导、丛生芽的增殖以及生根诱导进行了系统的研究。结果表明：升汞对除虫菊种子的毒害作用太大，次氯酸钠对种子的消毒效果比较好。叶片消毒选择次氯酸钠8 min为宜，成活率高达95%；不定芽诱导的最适培养基为MS+6-BA 0.5 mg/L+NAA 0.05 mg/L，诱导率最高可达83.3%，不定芽个数为8.15；丛生芽增殖的最适培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L，增倍数达9.16；生根的最适培养基为不加激素的MS，生根率为80%。
- Abstract:
- Using pyrethrum cinerariaefolium leaves as experimental materials，the factors of adventitious buds induction，shoot buds proliferation and shoots rooting were investigated systematically.The results showed that the effects of leaf sterilized with sodium hypochlorite were suitable than those with mercuric chloride.Leaf was sterilized in choice sodium hypochlorite for 8 minutes and the survival rate reached as high as 95%；The suitable medium of adventitious buds induction was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L，which made the shoot buds differentiation frequency of 83.3% and the average number of shoot buds per explants was 8.15.The shoots proliferation medium was MS+6-BA 1.0 mg/L+NAA 0.5mg/L，with the ratio of buds propagation was 9.16.The optimal rooting medium was MS without no hormones and the rooting rate of 80%.
参考文献/References:
[1] 陈宗莲 , 侯岁稳 , 俞宏渊 . 除虫菊的组织培养 [J]. 云南植物研究 ,1998,20(3):351-354. [2]杜冰群,刘启宏,朱翠英.除虫菊的染色体数目及其核型[J].武汉植物学研究,1988,6(1):95. [3]陈寿宏.真空冻干技术在除虫菊鲜花干燥中的应用研究[J].农药科学与管理,2003,24(9):31-33. [4]刘蓁,高山林.白花除虫菊组织培养研究[J].药物生物技术,2005,12(6):370-374. [5]吴沿友,张平夫.除虫菊组织培养研究[J].贵州科学,1995,13(3):28-30. [6]任秋萍,张复君.驱蚊草的栽培管理技术[J].农业新科技,2002(3):11-12. [7]顾刘金,杨校华,陈琼姜,等.天然除虫菊酯的免疫毒性[J].职业与健康,2003,19(12):37-38. [8]董建新,马志卿.除虫菊愈伤组织的诱导和继代[J].西北农林科技大学学报,2004,32(3):77-79. [9]任彦荣.除虫菊组织培养的研究[J].甘肃联合大学学报(自然科学版),2009,23(4):61-63. [10]张强,李琐,张兴.除虫菊组织培养中种子及幼苗消毒方法研究[J].陕西农业科学,2007(2):27-28,55. [11]张军云,马文彬,瞿观,等.除虫菊组织培养快繁技术研究[J].西南农业学报,2010,23(3):989-992. [12]郅慧,高山林.杭白菊茎尖组织培养及试管苗繁殖技术研究[J].植物资源与环境学报,2004,13(1):24. [13]李胜,李唯.植物试管苗玻璃化现象研究进展[J].甘肃农业大学学报,2003,3(1):4-19. [14]孙满芝,尹成涛.植物组培过程中玻璃化现象的发生与解决措施[J].山东林业科技,2001(6):19-20. [15]吴林,吴飞,余家平,等.驱蚊草组织培养及其愈伤组织诱导研究[J].生物学杂志,2007,24(4):45-48. [16]董倩,李凤玉.驱蚊草组织培养及其再生体系的建立与优化[J].福建师范大学学报,2006,22(1):73-76. [17]张新英,韩厉玲,李白玲.离体培养下白杜木质部的形态发生研究[J].植物学报,1989,31(7):489-494. [18]柯善强,傅俊,桂耀林,等.裂叶悬钩子器官发生的细胞组织学观察[J].植物学报,1989,31(11):889-891. [19]颜昌敬.植物组织培养手册[M].上海:上海科学技术出版社,1990. [20]徐振彪,傅作申,原亚萍,等.植物组织培养过程中的褐化现象[J].国外农学—杂粮作物,1997(1):55-56. [21]张俊琦,罗晓芳.牡丹组织培养中褐化的发生原因与防治方法的研究[J].沈阳农业大学学报,2006(5):720-724. [22]陈政,李程斌,何家宝,等.在正交设计在驱蚊草组织培养中的应用[J].安徽师范大学学报,2008,31:364-367. [23]李丽,何康.两种红豆杉植物的愈伤组织培养及褐化抑制[J].复旦学报(自然科学版),2006,45(6):705-707. [24]柴明良.留兰香玻璃苗愈伤组织化再生正常植株[J].植物生理学通讯,1991,27:371-376. [25]陈龙清,李春.日本早小菊的组织培养及玻璃苗防治[J].华中农业大学学报，1995,6(3):272-274. [26]黄宇翔.无病毒香石竹组培玻璃化控制研究[D].福州:福建农林大学,2005.
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备注/Memo
第一作者简介：于娅（1976-），女，博士，讲师，研究方向为植物遗传育种与基因工程。E-mail:yuy1025@163.com.