LI Wen-jie,PU Xiao-lan,CHEN Ze-ying.Study on in vitro Culture and Plant Regeneration of Menyanthes trifoliata[J].Northern Horticulture,2012,36(23):122-124.
睡菜的离体培养与植株再生研究
- Title:
- Study on in vitro Culture and Plant Regeneration of Menyanthes trifoliata
- 文章编号:
- 1001-0009（2012）23-0122-03
- Keywords:
- Menyanthes trifoliata L; callus; seedling; plant regeneration
- 分类号:
- S 682.32
- 文献标志码:
- A
- 摘要:
- 以不带节的睡菜茎段为外植体，研究其离体再生体系。结果表明：将茎段接种到MS+6-BA 1.0 mg/L+IBA 0.5 mg/L培养基上10 d可以诱导产生愈伤组织，然后转接至MS+6-BA 2.5 mg/L+NAA 0.2 mg/L+KT 0.05 mg/L培养基上15 d可使愈伤组织分化为幼苗；将带节的睡菜茎段接种到MS+IBA 0.1～0.2 mg/L培养基上7 d可使其腋芽萌发为幼苗；将幼苗接种到1/2MS+IBA 0.5 mg/L培养基上3～5 d可生根，实现植株再生。
- Abstract:
- Taking the stems without axillary bud of Menyanthes trifoliate as explants，its in vitro culture and regeneration were studied.The results showed that putting the stem on the medium MS+6-BA 1.0 mg/L+IBA 0.5 mg/L for 10 d could produce callus，then put them on MS+6-BA 2.5 mg/L+NAA 0.2 mg/L+KT 0.05 mg/L for 15 days，it could differentiate into seedlings without root；taking the stems with bud on MS+IBA 0.1～0.2 mg/L for 7 days，axillary bud could germinate and then be seeding without root.Put the seedling on 1/2MS+IBA 0.5 mg/L for 3~5 d，it could rooting.
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备注/Memo
第一作者简介：李文洁（1982-），女，云南人，在读硕士，研究方向为植物生物技术。E-mail:cmhm920@163.com.