QU Ling,SHI Zhi-gang,JIAO En-ning,et al.Effect of the Filtered Solution of Colletotrichum gloeosporioides Penz.on the Growth and Differentiation of Callus in Lycium barbarum L.[J].Northern Horticulture,2012,36(23):112-115.
枸杞炭疽病菌滤液对枸杞愈伤组织生长及分化的影响
- Title:
- Effect of the Filtered Solution of Colletotrichum gloeosporioides Penz.on the Growth and Differentiation of Callus in Lycium barbarum L.
- 文章编号:
- 1001-0009（2012）23-0112-04
- Keywords:
- Colletotrichum gloeosporioides Penz.; culture filtrate; callus; selection method and pressure; toxic effect
- 分类号:
- S 436.639
- 文献标志码:
- A
- 摘要:
- 采用Czapek-Dox培养液，通过振荡培养、过滤、离心，获得枸杞炭疽病菌（Colletotrichum gloeosporioides Penz.）培养滤液，配制含不同比例粗毒素液的枸杞愈伤组织培养与分化培养基，研究培养滤液对枸杞叶片愈伤组织生长和分化的影响。结果表明：枸杞炭疽病菌粗毒素液具有较强的生物活性，对枸杞愈伤组织生长及分化均具有一定的毒害和抑制作用；多步正选择法与一步正选择法相比，经过由低到高的选择压力逐级筛选后，可相对提高叶片愈伤组织对毒素的抗性，抗性细胞系筛选的上限浓度可定为浓度50%(v/v)，抗性细胞变异系分化培养基中的粗毒素液含量可定为20%(v/v)。
- Abstract:
- The effect of the filtered solution of Colletotrichum gloeosporioides Penz. on the growth and differentiation of calli in Lycium bararum L.were studied.The results indicated that the crude toxin had toxic effect and evidently inhibited the growth and differentiation of calli in Lycium barbarum L.in vitro.Compared with one-step screening system，the resistance of callus to Lycium barbarum L.toxin could be improved by increasing the concentration gradually in multi-step screening system.The toxin concentration of 50% could be considered as the selection pressure of resistant cell line and the concentration of 20% could be regarded as the screen concentration of differentiation of resistant cell line to Colletotrichum gloeosporioides Penz.
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备注/Memo
第一作者简介：曲玲（1972-），女，宁夏银川人，硕士，副研究员，现主要从事枸杞抗病性和枸杞生物技术抗病育种研究工作。