SUN Yong-jian,SUN Ning,CAO Zhi-pan,et al.Study on DNA Extraction of Saintpaulia ionantha for AFLP Reaction System[J].Northern Horticulture,2012,36(22):104-107.
适于AFLP分析的非洲紫罗兰DNA提取方法研究
- Title:
- Study on DNA Extraction of Saintpaulia ionantha for AFLP Reaction System
- 文章编号:
- 1001-0009（2012）22-0104-04
- Keywords:
- DNA extraction; Saintpaulia ionantha; CTAB; freeze-drying; AFLP analysis
- 分类号:
- S 681.2
- 文献标志码:
- A
- 摘要:
- 以非洲紫罗兰同一植株的叶片和茎段为试材，采用新鲜组织提取、烘干组织提取、冷冻干燥组织提取的3种不同预处理方法，并采用DNA Kit、CTAB-Ⅰ、CTAB-Ⅱ 3种提取方法，比较分析了DNA片断大小及不同方法提取DNA的纯度与得率，以期确定适用于多酚、多糖、水分含量高的非洲紫罗兰的DNA提取方法。结果表明：采用冷冻干燥组织提取法，经改良的CTAB-Ⅱ法，即在2次抽提步骤中，加入一步利用CTAB沉淀液沉淀DNA的方法最适合非洲紫罗兰DNA提取。这样得到的基因组DNA可作为后续分子水平研究的优良PCR模板。
- Abstract:
- Taking the same leaves and stems of Saintpaulia ionantha as materials,fresh tissue extraction,drying tissue extraction,freeze drying tissue extraction,3 different pre-treatment were adapted,also DNA Kit,CTAB-Ⅰ,CTAB-Ⅱ 3 different extraction methods were adopt,to compare DNA fragment size,purity and yield throagh different extraction method.The best DNA extraction method for Saintpaulia ionantha which contained more polysaccharide polyphenols and moisture was determined.The results showed CTABⅡ method and the material processed by freeze-drying before test,namely,in the second extraction steps,added CTAB preeipitation in the method of DNA precipitation methed.DNA received by this method could be used as template for PCR in study at further molecular research.
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备注/Memo
第一作者简介：孙永健（1983-），男，硕士，助教，现主要从事生物化学及分子生物学方面的教学与研究工作。E-mail：syjwonderful@yahoo.com.cn.