ZHAO Li-hua.Establishment of the Optimized AFLP Analysis System for Pomegranate[J].Northern Horticulture,2012,36(21):79-82.
石榴AFLP反应体系的建立及优化
- Title:
- Establishment of the Optimized AFLP Analysis System for Pomegranate
- 文章编号:
- 1001-0009（2012）21-0079-04
- Keywords:
- pomegranates; AFLP; system; optimized
- 分类号:
- S 665.4
- 文献标志码:
- A
- 摘要:
- 以提取的‘粉红牡丹’石榴叶片基因组DNA为材料，对影响AFLP反应体系的Mse I/EcoRⅠ酶切时间、预扩增模板DNA稀释倍数及程序和选择性扩增模板DNA稀释倍数等关键因素进行优化。结果表明：在石榴AFLP反应体系为20 μL双酶切反应体系中，基因组DNA为300 ng的最佳酶切时间为6 h；以不稀释DNA连接产物为预扩增模板，94℃为预扩增反应程序的起始解链温度；以稀释20倍预扩产物DNA为选扩模板。
- Abstract:
- Taking genome DNA of pomegranate ‘Fenhongmudan’ as material，AFLP reaction system was optimized from time of Mse I/EcoRⅠ restriction enzyme digest system，template DNA dilution multiple and program of pre-amplification，template DNA dilution multiple of selective amplification.The results indicated that 300 ng genomic DNA was digested completely with 6 hours，the products of ligation was not diluted；starting melting temperature was 94℃ for pre-amplification；the products of pre-amplification was diluted for 20 times.
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备注/Memo
作者简介：赵丽华（1972-），女，四川西昌人，博士，副教授，研究方向为园艺植物分子生物学及遗传育种。E-mail:1973zlh@163.com.