[1]王华宇,张树明,何贵整.大滨菊离体快繁技术研究[J].北方园艺,2012,36(19):143-145.
WANG Hua-yu,ZHANG Shu-ming,HE Gui-zheng.Study on Rapid Propagation in vitro of Leucanthemum maximum[J].Northern Horticulture,2012,36(19):143-145.
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WANG Hua-yu,ZHANG Shu-ming,HE Gui-zheng.Study on Rapid Propagation in vitro of Leucanthemum maximum[J].Northern Horticulture,2012,36(19):143-145.
大滨菊离体快繁技术研究
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第36卷
期数:
2012年19期
页码:
143-145
栏目:
出版日期:
2012-10-30
- Title:
- Study on Rapid Propagation in vitro of Leucanthemum maximum
- 文章编号:
- 001-0009(2012)19-0143-03
- Keywords:
- Leucanthemum maximum; aseptic shoots; tissue culture
- 分类号:
- S 681.9
- 文献标志码:
- A
- 摘要:
- 以大滨菊的茎段为外植体,对无菌芽诱导、试管苗增殖、生根及移栽技术进行了系统研究。结果表明:大滨菊腋芽诱导的最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.05 mg/L;最适宜的继代培养基为MS+6-BA 0.3 mg/L+NAA 0.03 mg/L;生根的最适宜培养基为1/2MS+IBA 0.5 mg/L。继代周期25 d左右,生根需要15 d,生根率可达95%以上,移栽成活率达到100%。
- Abstract:
- The stems of Leucanthemum maximum were used as explants,the aseptic shoots induction,propagation of the plants in vitro,rooting and transplanting were studied.The results showed that the best media for inducing new buts was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L;the best media for propagationg was MS+6-BA 0.3 mg/L+NAA 0.03 mg/L;the best media for rooting was 1/2MS+IBA 0.5 mg/L.The proper subculture cycle was about 25 days and the rooting stage took about 15 days.The rooting rate was as much as 95% and the survival rate of transplanting was 100%.
参考文献/References:
[1]中国植物志编委会.中国植物志[M].北京:科学出版社,1983. [2]河北植物志编委会.河北植物志[M].石家庄:河北科学技术出版社,1991. [3]茹先古丽·克依木.大滨菊在库尔勒地区的常规栽培技术[J].农村科技,2009(4):88-89. [4]杨广乐,宋兆,王军.北方园林露地草本花卉新品种-“大花滨菊”的栽培及应用[J].北方园艺,1994(2):43. [5]谭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社,1991. [6]崔继梅,梁艳丽,谢世清.魔芋组培快繁中常见的几个问题及对策[J].云南农业大学学报,2008,23(1):96-98. [7]孙庆春,郑成淑,丰震.菊花玻璃化苗与正常苗的生理特性比较[J].山东农业科学,2009(5):45-47.
备注/Memo
第一作者简介:王华宇(1981-),男,硕士,工程师,现主要从事植物组织培养和园艺栽培学研究工作。E-mail:hywangsky@163.com.责任作者:何贵整(1966-),男,高级工程师,现主要从事植物组织培养和引种及栽培研究工作。E-mail:guizhenghe@sohu.com.
更新日期/Last Update:
2014-09-09