ZHANG Xiao-li,LIANG Ling-ling,ZHAN Ruo-ting,et al.Research on Tissue Culture and Plant Regeneration of Lingnan Genuine Medicinal Materials Nervilia fordii[J].Northern Horticulture,2012,36(19):140-142.
岭南道地药材青天葵的组织培养与植株再生
- Title:
- Research on Tissue Culture and Plant Regeneration of Lingnan Genuine Medicinal Materials Nervilia fordii
- 文章编号:
- 1001-1009（2012）19-0140-03
- Keywords:
- Nervilia fordii; corm; tissue culture; regeneration plant
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以青天葵新鲜球茎为试材，对离体条件下青天葵的愈伤组织诱导及其植株再生进行研究。结果表明：0.1%升汞溶液处理青天葵球茎10 min、玻璃培养皿有利于青天葵愈伤组织的诱导；青天葵走茎及愈伤组织在MS+1.0 mg/L 6-BA培养基中培养可得到大量丛生芽及愈伤组织，多次继代培养可得到完整植株；将青天葵走茎接种于含有0.1%活性炭的MS培养基中可获得大量组培球茎。
- Abstract:
- Taking fresh corm of Nervilia fordii (Hance) Schltr.as material，the technique of callus induction and plantlet regeneration were studied.The results showed that Nervilia fordii corm treated by 0.1% HgCl2 solution for 10 min，glass culture dish were in favor of the induction and regeneration of callus.High frequency multiple shoot-bud formation from callus induced by Nervilia fordii corm inducing culture on a MS-medium supplemented with 1.0 mg/L 6-BA.After several times of successive transfers of culture，regenerated plant could be obtained.The optimum medium for the induction of corm was a MS-medium contains 0.1% activated charcoal.
参考文献/References:
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备注/Memo
第一作者简介：张晓丽（1987-），女，在读硕士，研究方向为中药资源的可持续利用研究和开发。E-mail:gzyxiaoli_2010@163.com.