CHEN Guo-liang,CHEN Zong-li,SUN Ping,et al.Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves[J].Northern Horticulture,2012,36(19):131-133.
枣树幼叶总RNA提取方法的比较研究
- Title:
- Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves
- 文章编号:
- 1001-0009（2012）19-0131-03
- Keywords:
- young jujube leaves; total RNA; extraction method; electrophoresis
- 分类号:
- Q 75
- 文献标志码:
- A
- 摘要:
- 以枣树幼嫩叶片为试材，分别采用DEPC水法、SDS抽提法、CTAB-LiCl和异硫氰酸胍法4种方法进行总RNA的提取试验，并对其进行了紫外、变性琼脂糖凝胶电泳及RT-PCR检测。结果表明：4种提取方法中DEPC水法提取的总RNA质量最好，SDS抽提法和CTAB-LiCl法次之，异硫氰酸胍法效果最差。
- Abstract:
- Taking young jujube leaves as material，RNA in jujube leaves was extracted by the DEPC water method，SDS method，CTAB-LiCl method and isothiocyanate guanidine method.The quality of the total RNA was tested by nuclear acid/protein analyzer，denaturation agarose gel electrophoresis and RT-PCR.The results showed that total RNA extracted by DEPC water method had the best quality and reliability，followed by SDS extraction method and CTAB-LiCl method，the isothiocyanate guanidine method was the worst.
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备注/Memo
第一作者简介：陈国梁（1974-），男，陕西定边人，硕士，讲师，研究方向为植物生物技术。E-mail：glc9359@163.com.