ZHAO Zhi-chang,HU Fu-chu,HUANG Jian-feng,et al.Cloning and Aequence Analysis of ANS Gene from Litchi chinensis[J].Northern Horticulture,2012,36(19):118-121.
荔枝ANS基因的克隆及其序列分析
- Title:
- Cloning and Aequence Analysis of ANS Gene from Litchi chinensis
- 文章编号:
- 1001-0009（2012）19-0118-04
- Keywords:
- Litchi chinensis Sonn.; ANS; gene cloning; sequence analysis
- 分类号:
- S 667.1
- 文献标志码:
- A
- 摘要:
- 以3个不同发育时期的‘糯米糍’荔枝果皮和幼嫩叶片为试材，对其ANS基因进行了克隆及分析。结果表明：花青素合成酶 （Anthocyanidin synthase，ANS） 是花色素苷生化合成途径中的一个关键酶。利用同源克隆方法从荔枝果皮中克隆得到了1个ANS基因，该基因开放阅读框的长度为1 074 bp，编码357个氨基酸。以基因组为模板，扩增得到了1 279 bp的核苷酸序列与cDNA序列比对发现，基因组序列中还有1个内含子，位置在504~708 bp之间。通过系统发育分析发现，该基因编码的蛋白与葡萄、可可豆、柑橘等聚为一类。
- Abstract:
- Taking the peel and young leaves of Litechi chinensis ‘Nuomici’ at three different stages as material,its ANS gene was cloned and analyzed.The results showed that the anthocyanidin synthase (ANS) was a key enzyme in the anthocyanin biosynthesis.A ANS gene was cloned from litchi pericarp by homologous cloning method.The open reading frame was 1 074 bp，and encoding 357 amino acids.The 1 279 bp length fragment was amplified from genome and analysis，which contained five introns，respectively，in 504~708 bp.It was found that the gene encoding for the protein had close relationship with mountain grape (Vitis vinifera)，cocoa (Theobroma cacao)，citrus (Citrus sinensis)，and other fruit trees through phylogenetic analysis.
参考文献/References:
[1]李建国.荔枝学[M].北京：中国农业出版社，2008:2-3. [2]吴淑娴.中国果树志·荔枝卷[M].北京：中国林业出版社，1998. [3]Xie D Y，Jackson L A，Cooper J D，et al.Molecular and biochemical analysis of two cDNA clones encoding Dihydroflavonol-4-Reductase from Medicago truncatula?[J].Plant Physiology,2004,134:979-994. [4]Rosati C，Cadic A，Duron M，et al.Molecular characterization of the anthocyanidin synthase gene in Forsythia×intermedia reveals organ-specific expression during flower development [J].Plant Science,1999,149:73-79. [5]Jaakola L，Maatta K，Pirttila A M，et al.Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin，proanthocyanidin，and flavonol levels during bilberry fruit development [J].Plant Physiology,2002,130:729-739. [6]赵志常,胡桂兵,刘运春,等.番荔枝DNA的提取和AFLP体系的建立[J].北方园艺,2009(10):44-47. [7]Bugos R C,Chiang V L，Zhang X，et al.RNA isolation from plant tissues recalatrant to extraction in guanidine[J].Biotechniques,1995,19:734-737. [8]Lu Y Q，Rausher M D.Evolutionary rate variation in anthocyanin pathway genes [J].Molecular Biology and Evolution,2003,20(11):1844-1853.
相似文献/References:
[1]刘 忠,黄 娇.乐山荔枝主要虫害种类及发生规律的研究[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007062]
LIU Zhong,HUANG Jiao.Studies on the Main Species andOccurrence Characteristics of Litchi Pests in Leshan[J].Northern Horticulture,2010,34(19):0.[doi:10.11937/bfyy.201007062]
[2]曾小红,张慧坚,谢龙莲,等.荔枝遗传基础研究进展[J].北方园艺,2016,40(06):185.[doi:10.11937/bfyy.201606048]
ZENG Xiaohong,ZHANG Huijian,XIE Longlian,et al.Research Progress on Gentics Basis of Litchi chinensis Sonn.[J].Northern Horticulture,2016,40(19):185.[doi:10.11937/bfyy.201606048]
[3]姜 仲 书,江 国 良,张 光 伦.四川荔枝栽培技术要点[J].北方园艺,2008,32(04):0.[doi:10.11937/bfyy.200804057]
[J].Northern Horticulture,2008,32(19):0.[doi:10.11937/bfyy.200804057]
备注/Memo
第一作者简介：赵志常（1977-），男，山东烟台人，博士，副研究员，现主要从事热带果树的遗传育种工作。