ZHU Hong-fen,SHEN Lan,HUANG Jian,et al.Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’[J].Northern Horticulture,2012,36(19):105-107.
兔眼蓝莓“灿烂”组织培养与植株再生研究
- Title:
- Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’
- 文章编号:
- 1001-0009（2012）19-0105-03
- Keywords:
- Vaccinium ashei; tissue culture; plant regeneration
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以兔眼蓝莓“灿烂”（‘Britewell’）当年生带腋芽半木质化茎段为外植体，研究了不同培养基、激素对兔眼蓝莓“灿烂”植株再生的影响。结果表明：WPM为适宜基本培养基；WPM+（0.5～1.0）mg/L ZT组合是最适的初代幼芽诱导培养基，诱导率达86%以上；WPM+0.1 mg/L TDZ+0.1 mg/L IBA组合是较合适的增殖培养基，增殖率可达5.48，继代培养宜以较低浓度的TDZ（0.02 mg/L和0.05 mg/L）交替使用为好；1/2WPM+1.0 mg/L 6-BA是较理想的生根培养基组合，有效生根率达63.2％。
- Abstract:
- Taking semi-lignified stem with axillary bud of Vaccinium ashei ‘Britewell’ as explants，the effects of the different medias and hormones on plant regeneration were studied.The results showed that WPM was a suitable basic medium.The optimal primary medium for stem with axillary bud was WPM+(0.5～1.0) mg/L ZT，and the induction rate was more than 86%；the suitable proliferation medium was WPM+0.1 mg/L TDZ+0.1 mg/L IBA，the proliferation rate up to 5.48 times.Following the subculture should be at a lower concentration of TDZ (0.02 mg/L and 0.05 mg/L) alternating as well；the rooting medium was 1/2WPM+1.0 mg/L BA，and the effective rooting rate reached 63.2％.
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备注/Memo
第一作者简介：朱宏芬（1974-），女，浙江宁波人，本科，农艺师，现主要从事植物组培研究工作。E-mail：nbnkyzhu@163.com.