[1]彭广霖,于咏梅,薛元霞,等.氯化汞防治组培苗污染的研究[J].北方园艺,2012,36(15):131-133.
PENG Guang-lin,YU Yong-mei,XUE Yuan-xia,et al.Research on the Prevention of Tissue Culture Seedling Pollution by Mercuric Chloride[J].Northern Horticulture,2012,36(15):131-133.
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PENG Guang-lin,YU Yong-mei,XUE Yuan-xia,et al.Research on the Prevention of Tissue Culture Seedling Pollution by Mercuric Chloride[J].Northern Horticulture,2012,36(15):131-133.
氯化汞防治组培苗污染的研究
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第36卷
期数:
2012年15期
页码:
131-133
栏目:
出版日期:
2012-08-15
- Title:
- Research on the Prevention of Tissue Culture Seedling Pollution by Mercuric Chloride
- 文章编号:
- 1001-0009(2012)15-0131-03
- 分类号:
- Q 943.1
- 文献标志码:
- A
- 摘要:
- 以大花萱草和切花菊组培苗为试材，在MS培养基中分别加入0.005、0.010、0.030、0.050、0.070 mg/L 氯化汞，研究抑菌剂氯化汞对污染组培苗的消除污染效果。结果表明:当浓度在0.010 mg/L时，经过2、3次的转接后，能明显地消除污染。当浓度在0.030 mg/L时,1次转接即能对污染起到较好的抑制效果，并且随浓度的增加，抑制效果越好。但浓度超过0.080 mg/L时，组培苗迅速出现脱绿死亡现象。综合分析认为，氯化汞的浓度在0.030~0.070 mg/L是防治大花萱草和切花菊组培苗污染的最佳浓度。
- Abstract:
- Chosen hemerocallis and cut chrysanthemum seedlings as materials，mercuric chloride at the rate of 0.005,0.010,0.030,0.050 and 0.070 mg/L was added into culture medium,respectively,the effects of mercuric chloride on the pollution prevention of tissue culture seedling were studied.The results showed that mercuric chloride could significantly eliminate pollution at 0.010 mg/L concentration by 2 to 3 switches.As mercuric chloride contents were between 0.030 and 0.070 mg/L，the pollution was markedly inhibited and a positive relationship was found between inhibition effect and concentrations.0.080 mg/L mercuric chloride was unfavorable for tissue culture seedlings indicted by leaf chlorosis and plant death.It was concluded that concentrations between 0.030 and 0.070 mg/L may be the better choice to prevent pollution for hemerocallis and cut chrysanthemum seedlings.
参考文献/References:
[1]田新平.培养基污染防治方法研究[J].现代农业科技,2010(13):11-13. [2]于福科,张广军.玫瑰组织培养污染控制技术措施[J].陕西农业科学,2002(11):47-48. [3]周俊辉,周厚高,刘花全.植物组织培养中的内生细污染问题[J].广西植物,2003(23):41-47. [4]许婉芳,龚福生,萧华山.杀菌剂对金线莲组织培养中微生物污染的抑制作用[J].福建果树,1999(4)：6-7.
备注/Memo
第一作者简介：彭广霖（1959-）,男,本科,讲师,现主要从事农业经济及作物栽培等研究工作。E-mail：ytpgl1959@163.com.责任作者：史淑一（1964-），女，本科，高级讲师，现主要从事应用气象研究工作。E-mail：ssy19640723@yahoo.com.cn.基金项目：中国农业大学（烟台）校级课题资助项目（yt2008.16）。
更新日期/Last Update:
2014-08-28