WEI Kun-hua,LI Lin-xuan,WANG Mei-ying,et al.Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.[J].Northern Horticulture,2012,36(13):185-187.
山豆根组培苗的质量分析
- Title:
- Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.
- 文章编号:
- 1001-0009(2012)13-0185-03
- 分类号:
- S 567.1+9
- 文献标志码:
- A
- 摘要:
- 以山豆根组培生根苗为试材，采用TLC法鉴别其是否存在苦参碱和氧化苦参碱，并使用HPLC法测定其组培苗移栽前后苦参碱和氧化苦参碱的含量。结果表明：山豆根组培生根苗含有苦参碱和氧化苦参碱，且与氧化苦参碱和苦参碱对照品色谱相同的位置上，显示相同的橙红色斑点；HPLC定量分析结果表明，山豆根组培苗移栽田间4个月含有的苦参碱和氧化苦参碱均低于田间生长6个月的山豆根实生苗。
- Abstract:
- With Sophora tonkinensis Gapnep tissue culture seedling as material,the presence of matrine and oxymatrine in the tissue-cultured plantlets of Sophora tonkinensis Gapnep.were identified by TLC，and also the matrine and oxymatrine contents of tissue-cultured plantlets before and after transplanted were determinated by HPLC.The results showed that there were reddish orange spots at the corresponding place of matrine and oxymatrine.The analysis of HPLC showed that both of the tissue-cultured plantlets before transplanted and after transplanted in the field for four monthes possessed matrine and oxymatrine，but lower than the seedlings from seeds planted in the field for half year.This study demonstrates that the tissue-cultured plantlets of S.tonkinensis Gapnep.contain matrine and oxymatrine，and it will provide a good support for follow-up research.
参考文献/References:
[1]中华人民共和国国家药典委员会.中华人民共和国药典(2010版)[M].北京:中国医药科技出版社,2010.
[2]覃文流,凌征柱,许鸿源,等.山豆根组织培养获得再生植株[J].中国中药杂志,2005,30(4):303-304.
[3]覃文流,凌征柱,许鸿源,等.广豆根组培苗与野生植株有效成分的对比分析[J].中药材,2004,27(8):552-553.
[4]Huang H P，Gao S L，Chen L L，et al.In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis[J].Pharmacognosy Magazine，2010,6(21):51-56.
[5]Wei K H，Miao J H，Huang H P，et al.Generation of autotetraploid plant of ginger (Zingiber offcinale Rosc.) and its quality evaluation [J].Pharmacognosy Magazine,2011,7(27):200-206.
[6]赵磊磊,谢凯,朱盛山,等.不同产地丹参的HPLC和TLC图谱分析[J].中药材,2007,30(6):646-648.
相似文献/References:
[1]姚 绍 嫦,凌 征 柱,蓝 祖 栽,等.山豆根组培快繁技术优化的研究[J].北方园艺,2011,35(06):0.[doi:10.11937/bfyy.201106048]
YAO Shao -chang,LING Zheng -zhu,LAN Zu -zai,et al.Optimization of Tissue Culture on Sophora tonkinensis Gapnep.[J].Northern Horticulture,2011,35(13):0.[doi:10.11937/bfyy.201106048]
[2]梁莹,邓传华,蔡锦源,等.不同施肥配比对山豆根生长及有效成分的影响[J].北方园艺,2019,43(12):108.[doi:10.11937/bfyy.20183638]
LIANG Ying,DENG Chuanhua,CAI Jinyuan,et al.Effects of Different Fertilization Ratios on the Growth and the Effective Constituent of Sophora tonkinensis Gagnep.[J].Northern Horticulture,2019,43(13):108.[doi:10.11937/bfyy.20183638]
[3]蓝芳,向维,张平刚,等.不同种植密度对山豆根生长及有效成分的影响[J].北方园艺,2017,41(04):140.[doi:10.11937/bfyy.201704033]
LAN Fang,XIANG Wei,ZHANG Pinggang,et al.Effects of Different Planting Densities on the Growth and the Effective Constituent of Sophora tonkinensis [J].Northern Horticulture,2017,41(13):140.[doi:10.11937/bfyy.201704033]
备注/Memo
第一作者简介：韦坤华（1983-），女，博士，助理研究员，现主要从事中药生物技术等研究工作。
责任作者：缪剑华（1961-），男,博士，研究员，现主要从事药用植物中药学等研究工作。
基金项目：国家发改委中药材扶持资金资助项目（发改运行[2007]2706号）；广西壮族自治区自然科学基金资助项目（桂科自0991025Z）。
收稿日期：2012-04-23