YANGJing .,CAOHai-feng.Determinationof14OrganPhosphorusinCucumberof GreenVegetablesbyUsingGasChromatography[J].Northern Horticulture,2012,36(12):25-28.
利用气相色谱法检测绿色蔬菜黄瓜中十四种有机磷农药残留
- Title:
- Determinationof14OrganPhosphorusinCucumberof GreenVegetablesbyUsingGasChromatography
- 文章编号:
- 1001-0009（2012）12-0025-04
- Keywords:
- greenvegetable; cucumber; gaschromatography; organphosphorus
- 分类号:
- Q946.91
- 文献标志码:
- A
- 摘要:
- 选用安捷伦7890A-FPD，DB-1701（30m×0.25mm×0.25μm）毛细管色谱柱分离，样品用乙腈提取，氮吹水浴浓缩温度为70℃，建立了14种有机磷农药在绿色食品蔬菜黄瓜中的残留分析方法。结果表明：14种农药在0.01~1.0mg/kg范围内线性良好，相关系数为0.9978～0.9998，在23min内能够完全分离；14种有机磷农药的回收率均在65%～120%范围内，其相对标准偏差均低于5%，以3倍信噪比计算出的检出限均低于日本肯定列表限量要求。该方法适用于绿色食品蔬菜中14种有机磷农残的同时检测。
- Abstract:
- SelectedAgilent7890A-FPD,DB-1701（30m×0.25mm×0.25um）capillarycolumn,extractedwithacetonitrile,concentrationtemperaturewas70℃withnitrogenblow.Ananalyticalmethodforthedeterminationoforganphosphorusincucumberofgreenvegetablesbyusinggaschromatographywasestablished.Theresultsshowedthatthe14organphosphorus’slinearityweregood,theircorrelationcoefficientwerefrom0.9978to0.9998inrange0.０1~1.０mg/kgandtheycouldbeseparatedwithinthe23min;Atthesametimethatthe14organphosphorus’srecoverywerefrom65%to120%,andRSD%lessthan5%,detectionlimitlessthanlimitsofJapanesePositiveListSystem.Theconclusionwasthatthismethodwassuitablefordetecting14organphosphorusincucumberofgreenvegetablesatthesametime.
参考文献/References:
[1]杨艳玉,范三红,薛平,等.气相色谱法检测黄瓜中的48种有机磷农药残留的方法研究术[J].食品工程,2008(4):46-49.
[2]应兴华,徐霞,胡敏骏,等.毛细管气相色谱法测定韭菜中13种有机磷农药残留的研究[J].分析测试学报,2009,28(10):1185-1188.
[3]李文龙,单宝丽,依桂华,等.毛细柱气相色谱法快速测定黄瓜中六种有机磷农药残留[J].黑龙江八一农垦大学学报,2009,21(4):42-44.
[4]马丽娜,邵彦来,何贶,等.气相色谱法测定黄瓜中9种有机磷农药残留的方法研究[J].农药科学与管理,2006,25(7):18-22.
[5]李二虎,胡敏,吴兵兵,等.气相色谱法测定黄瓜中啶虫脒农药残留[J].农药,2006,45(7):479-480.
[6]周艳明,关丽,牛森,等.气相色谱法测定蔬菜中克菌丹、灭菌丹、敌菌丹残留量的研究[J].食品科学,2009,30(12):187-189.
[7]邬元娟,陈子雷,岳辉.气相色谱法测定黄瓜中哒螨灵残留量[J].农药,2008,47(5):363-364.
[8]徐存宽.简述气相色谱仪的检出限[J].粮油食品科技,2009,17(5):36-37.
[9]张有会,杨秀培,史兵方,等.气相色谱法测定蔬菜中有机磷农药残留的前处理方法比较[J].云南环境科学,2006,25(2):47-49.
[10]徐陆妹,楼冰冰.蔬菜中有机磷和氨基甲酸酯类农药的检测方法研究[J].中国卫生检验,2007(5):852-853.
[11]张贵群,聂思桥,龙丽萍,等.气相色谱法分析甘蓝及其土壤中的烯啶虫胺残留[J].色谱,2010,28(11):1103-1106.
[12]王建华,张艺兵,汤志旭,等.微波处理—气相色谱法测定含硫蔬菜中的多类农药残留[J].分析测试学报,2005,24(1):100-102.
[13]姜俊,李安,李海燕,等.大葱中25种有机磷农药多残留的微波净化-气相色谱测定[J].色谱,2007,25(3):395-398.
[14]贾斌,李瑾,冯书慧,等.微波处理-气相色谱法快速测定葱蒜类蔬菜中氟环唑残留[J].农药,2009,48(6):442-443.
[15]中华人民共和国国家标准.食品中农药最高残留限量标准[S].GB18406.1-18406.2-2001.
相似文献/References:
[1]李亚芹,郑根昌.冬棚黄瓜的氮磷钾肥配施效应分析[J].北方园艺,2014,38(12):146.
LI Ya-qin,ZHENG Gen-chang. Effect Analysis of Application of N,P and K Fertilizers on Cucumber in Winter Plastic Greenhouses [J].Northern Horticulture,2014,38(12):146.
[2]由玉卿,秦智伟,周秀艳,等.黄瓜果实中丙醇二酸含量的配合力分析[J].北方园艺,2014,38(10):19.
YOU Yu-qing,QIN Zhi-wei,ZHOU Xiu-yan,et al.Analysis on Combining Ability of Tartronic Acid Content in Cucumber[J].Northern Horticulture,2014,38(12):19.
[3]张秋萍,祁红英,张 珏,等.丛枝菌根制剂对黄瓜幼苗生长的影响[J].北方园艺,2014,38(14):37.
ZHANG Qiu-ping,QI Hong-ying,ZHANG Yu,et al.Effect of Arbuscular Mycorrhizal Agent on Growth of Cucumber Seedling[J].Northern Horticulture,2014,38(12):37.
[4]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(12):1.
[5]王昶童,曹刚强,梁丛敏,等.黄瓜NBS类抗病基因类似物的生物信息学分析[J].北方园艺,2014,38(14):101.
WANG Chang-tong,CAO Gang-qiang,LIANG Cong-min,et al.Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber[J].Northern Horticulture,2014,38(12):101.
[6]张瑞芬,李红岭,李新旭,等.优比胶囊不同使用方法对黄瓜生长及产量的影响[J].北方园艺,2013,37(17):4.
ZHANG Rui-fen,LI Hong-ling,LI Xin-xu,et al.Effect of Different Usage of UB Capsule on Growth and Yield of Cucumber[J].Northern Horticulture,2013,37(12):4.
[7]张雪艳,李堃,马丽娟,等.Ca2+调控对黄瓜幼苗耐盐性生理指标的影响[J].北方园艺,2013,37(13):5.
ZHANG Xue-yan,LI Kun,MA Li-juan,et al.Effects of Calcium Regulation on the Physiological Properties of Cucumber Under Salt Stress[J].Northern Horticulture,2013,37(12):5.
[8]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination，Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(12):9.
[9]张建祥,贾利元.越冬茬黄瓜结瓜期管理技术[J].北方园艺,2013,37(13):63.
ZHANG Jian-xiang,JIA Li-yuan.Management Techniques of Overwintering Cucumber During the Fruiting Period[J].Northern Horticulture,2013,37(12):63.
[10]裴倩如,朱本忠,田慧琴,等.冷激处理对黄瓜低温贮藏中冷害的影响[J].北方园艺,2014,38(01):127.
PEI Qian-ru,ZHU Ben-zhong,TIAN Hui-qin,et al.Effect of Cold Shock Treatment on Chilling Injury of Cucumber Under Low Temperature Storage[J].Northern Horticulture,2014,38(12):127.
备注/Memo
第一作者简介：杨静（1978-），女，在读博士，讲师，研究方向为蔬菜分子遗传育种，现主要从事蔬菜农药残留检测工作。E-mail：yangjkk@yahoo.com.cn。
收稿日期：2012-03-09