ZHANG Ling-ling,GUO Jun-zhan,ZHANG Min,et al.Study on Establishment of Aseptic System of Malus halliana[J].Northern Horticulture,2012,36(10):126-129.
垂丝海棠无菌体系建立研究
- Title:
- Study on Establishment of Aseptic System of Malus halliana
- 文章编号:
- 1001-0009（2012）10-0126-04
- Keywords:
- Malus halliana; explant disinfection; bud germination
- 分类号:
- S 685.99
- 文献标志码:
- A
- 摘要:
- 以垂丝海棠为试材,研究了外植体消毒的影响因素、芽诱导启动最适的培养基和植物生长激素浓度组合。结果表明：每年4~8月取材的幼嫩外植体灭菌效果最好,单芽茎段用75%酒精 30 s+0.1% HgCl2 消毒 6 min、叶片采用75%酒精 30 s+0.1% HgCl2消毒 8 min效果最佳，花瓣作为外植体,染菌率高,不利于无菌体系的建立；正交实验表明，芽诱导启动的最适培养基及激素浓度组合为：WPM+6-BA 1.0 mg/L+NAA 0.5 mg/L，启动率为87%。
- Abstract:
- Taking Malus halliana as test material,explant disinfection factors of Malus halliana and the best combination of culture medium and plant growth hormones concentration for bud germination were studied.The results indicated that the explants of best disinfectant effect were those which were young and sampled in April to August every year；the best disinfection method of stem segment with single bud was 75% alcohol 30 s with 0.1% HgCl2 6 min，the best disinfection method of leaf was 75% alcohol 30 s with 0.1% HgCl2 8 min，Because of high infection rate，it was too hard for Petal to establish aseptic system；Orthogonal experimental design showed that the best combination of culture medium and plant growth hormones concentration for bud germination was WPM+6-BA 1.0 mg/L+NAA 0.5 mg/L，and germination rate was 87%.
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相似文献/References:
[1]许 晓 岗,王 俊,童 丽 丽,等.垂丝海棠插穗生根与可溶性蛋白和激素变化的关系[J].北方园艺,2008,32(01):0.[doi:10.11937/bfyy.200801050]
XU Xiao -gang,W ANG Jun,TONG Li-li,et al.Studies on Changes of Soluble Proteins in the Cuttings Rooting of Malushalliana Roehne[J].Northern Horticulture,2008,32(10):0.[doi:10.11937/bfyy.200801050]
备注/Memo
第一作者简介：张玲玲（1988-），女，硕士，研究方向为园林植物遗传育种。E-mail:zhang.5527@163.com。
责任作者：郭军站（1963-），男，硕士，副教授，硕士生导师，研究方向为林木遗传育种。E-mail:guojunzhan@163.com。
收稿日期：2012-03-05