CHA Hui-ling,SHI Yu-ping,REN Shi-xia.Determination of Flavonoids Extraction in Soft Pear from Qinghai Province[J].Northern Horticulture,2012,36(10):42-44.
软儿梨果肉中黄酮类化合物的提取及含量测定
- Title:
- Determination of Flavonoids Extraction in Soft Pear from Qinghai Province
- 文章编号:
- 1001-0009(2012)10-0042-03
- Keywords:
- soft pear; flavonoids; single test; orthogonal test; extraction
- 分类号:
- Q 946
- 文献标志码:
- A
- 摘要:
- 以青海产软儿梨为试材，以乙醇为提取剂，通过单因素、正交实验对黄酮类化合物进行提取，研究其最佳提取条件。结果表明：提取的最佳条件为乙醇浓度95%，浸提温度为80℃，乙醇加入量为90 mL，浸提时间为3.0 h。以芦丁为标准物，通过分光光度法测定软儿梨果肉中总黄酮的含量为0.0189 g/100g。
- Abstract:
- With Qinghai soft pear as test materials，used ethanol as extracting agent，extracting flavonoids by single and orthogonal test were studied.The results showed that optimal conditions for extraction was 95% ethanol concentration，extraction temperature was high at 80℃，the amount of ethanol was up to 90 mL and the extraction time was 3.0 h.Using Rutin as the standard，the total content of flavonoids was 0.0189 g/100g in soft pear pulp by spectrophotometry.
参考文献/References:
[1]赵生元,宋柯.软儿梨果酒的研制[J].酿酒科技,2009,175(1):87-88.
[2]陆柏益,张英,吴晓琴.黄酮类化合物的潜在毒性作用[J].中国中药杂志,2006,31(7):533-536.
[3]Martin H D，Beutner S，Frixel S,et al.Modified flavonoidsas strong photoprotecting UV-Absorbers and Antioxdants [J].Chemische Vereniging,2003(1):288-291.
[4]蔡碧琼,余萍.稻壳中黄酮提取物结构的初步鉴定[J].湖北民族学院学报(自然科学版),2008,26(4):448-451.
[5]买合布白?阿不都热依木,艾克白尔,库尔班尼莎,等.心草中总黄酮含量的测定及其提取研究[J].生物技术,2007,17(4):67-69.
[6]葵健,王薇.黄瓜叶中总黄酮含量的研究[J].食品科学,2005,26(8):194-196.
[7]许钢,张虹,胡箭.竹叶中黄酮提取方法的研究[J].分析化学,2000(7):857-859.
[8]范志刚,麦军利.微波技术对雪莲中黄酮QIN出量影响的研究[J].中国民族医药杂志,2000(1):43-44.
[9]王选东,刘利林,许宗运.正交设计研究石榴皮总黄酮提取工艺[J].塔里木农垦大学学报,2003,15(2):11-15.
[10]华辉,郭勇.黄酮类化合物药理研究进展[J].广东药学,1999,9(4):9-12.
[11]王玮,王琳.黄酮类化合物研究进展[J].沈阳医学院学报,2001,4(2):115-119.
[12]古丽娜尔?夏依马尔旦.黄连中总黄酮含量测定及提取工艺的研究[J].新疆师范大学学报（自然科学版）,2010,29(1):77-78.
相似文献/References:
[1]何春霞.补血草属植物黄酮类化合物及药理作用研究进展[J].北方园艺,2013,37(04):182.
[2]杨桂珍,陈伟珍,冼彩霞,等.超声提取鼠曲草中水溶性黄酮类化合物和多糖工艺研究[J].北方园艺,2014,38(07):148.
YANG Gui-zhen,CHEN Wei-zhen,XIAN Cai-xia,et al.Study on Ultrasonic Extraction of Water-soluble Flavonoids and Polysaccharides From Gnaphalium affine[J].Northern Horticulture,2014,38(10):148.
[3]李红军.银杏叶中黄酮类化合物的提取及其体外抗氧化活性研究[J].北方园艺,2013,37(05):156.
LI Hong-jun.Study on Extraction of Flavonoids from Ginkgo biloba Leaves and Antioxidant Action in vitro[J].Northern Horticulture,2013,37(10):156.
[4]蒋钦任,马雅美,马永强,等.软儿梨冷冻贮藏过程生理指标的变化研究[J].北方园艺,2013,37(03):139.
JIANG Qin-ren,MA Ya-mei,MA Yong-qiang,et al.Study on Changes of Related Physiological Index of Ruaner Pear at Frozen Storage Process[J].Northern Horticulture,2013,37(10):139.
[5]王桂荣,张燕娣,张连学.响应面法优化飞机草总黄酮提取工艺[J].北方园艺,2012,36(15):78.
WANG Gui-rong,ZHANG Yan-di,ZHANG Lian-xue.Optimization of Extraction Technology for Total Flavonoids from Eupatorium odoratum L.by Response Surface Methodology[J].Northern Horticulture,2012,36(10):78.
[6]陈 英.软 儿 梨 枝 条 及 果 实 生 长 节 律 研 究[J].北方园艺,2011,35(18):0.
Ｓ ｔ ｕ ｄ ｙ ｏ ｎ ｔ ｈ ｅ Ｇ ｒ ｏ ｗ ｔ ｈ Ｒ ｈ ｙ ｔ ｈ ｍ ｏ ｆ ｔ ｈ ｅ Ｂ ｒ ａ ｎ ｃ ｈ ｅ ｓａ ｎ ｄ Ｆ ｒ ｕ ｉ ｔ ｏ ｆ Ｐ ｇ ｒ ｕ ｓ ｕ ｓ ｓ ｕ ｒ ｉ ｅ ｎ ｓ ｉ ｓ Ｍ ａ ｘ ｉ ｍ[J].Northern Horticulture,2011,35(10):0.
[7]王 虹.,任世霞,王永宁.软儿梨中氨基酸的提取及总氨基酸的定量分析[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108017]
WANG Hong.,PEN Shi-xiaI,WANG Yong-ningI.Extraction of Amino Acid and Quantitative Analysis of Total Amino Acid in ’ Qinghai’ Soft Pear[J].Northern Horticulture,2011,35(10):0.[doi:10.11937/bfyy.201108017]
[8]刘小利.套袋对两种地产梨果实品质的影响[J].北方园艺,2007,31(04):0.[doi:10.11937/bfyy.200704017]
LIU Xiao-li.Effect of Setting Bag on the Quality of Two Local Pear[J].Northern Horticulture,2007,31(10):0.[doi:10.11937/bfyy.200704017]
[9]朱建民,张国英,窦培培,等.三波长法测定超临界萃取九州虫草总黄酮含量[J].北方园艺,2017,41(02):132.[doi:10.11937/bfyy.201702032]
ZHU Jianmin,ZHANG Guoying,DOU Peipei,et al.Three-wavelength Ultraviolet Spectrophotometric Determination of Total Flavonoes From Cordyceps kyushuensis Extracted by Supercritical Fluid Method[J].Northern Horticulture,2017,41(10):132.[doi:10.11937/bfyy.201702032]
[10]梁志宏.软儿梨果实的品质及其加工相关研究综述[J].北方园艺,2018,42(04):180.[doi:10.11937/bfyy.20172410]
LIANG Zhihong.Review on Quality and Processing of Ruaner Pear Fruit[J].Northern Horticulture,2018,42(10):180.[doi:10.11937/bfyy.20172410]
备注/Memo
第一作者简介：查慧领（1965-），男，河南内黄人，本科，副教授，研究方向为分析化学。E-mail：qhwychl@163.com。
收稿日期：2012-02-17