[1]史伟国,张玉,吴宏斌,等.龙胆多糖最佳提取工艺研究[J].北方园艺,2012,36(09):181-183.
　SHI Wei-guo,ZHANG Yu,LIU Juan,et al.Study on the Technology of Homogenate Extraction of Gentiana Polysaccharide in Gentiana scabra[J].Northern Horticulture,2012,36(09):181-183.
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龙胆多糖最佳提取工艺研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年09期 页码: 181-183 栏目: 出版日期: 2012-09-16
Title:
Study on the Technology of Homogenate Extraction of Gentiana Polysaccharide in Gentiana scabra
文章编号:
1001-0009（2012）09-0181-03
作者:
史伟国1; 张玉1; 吴宏斌1; 宗希明1; 彭玉生1; 赵化启2
1.佳木斯大学 药学院，黑龙江省生物药制剂重点实验室，黑龙江 佳木斯 154007；
2.佳木斯大学 信息电子技术学院，黑龙江 佳木斯 154007
Author(s):
SHI Wei-guo1; ZHANG Yu1; LIU Juan1; ZONG Xi-ming1; WANG Li-hong1; DU Juan1; ZHAO Hua-qi2
1.College of Pharmacy of Jiamusi University,Key Laboratories of Heilongjiang Biological Medicine Preparations,Jiamusi,Heilongjiang 154007;
2.College of Information and Electronic Technology,Jiamusi University,Jiamusi,Heilongjiang 154007
关键词:
龙胆; 龙胆多糖; 匀浆提取; 正交实验
Keywords:
Gentiana scabra Bunge; Gentiana scabra Bunge polysaccharide; homogenate extraction; orthogonal experiment
分类号:
S 567.23+9
文献标志码:
A
摘要:
以龙胆为原料，采用正交设计的方法，以匀浆时间、匀浆次数、料液比为考察因素，对匀浆法提取龙胆中龙胆多糖工艺进行研究，确定其最佳工艺条件，为龙胆药用资源研究与开发提供可靠依据。结果表明：龙胆多糖提取最佳工艺参数为：匀浆时间9 min、匀浆次数3次、料液比1︰30(g︰mL)，在最佳条件下，龙胆多糖得率为12.59%。匀浆提取法操作简单、减少粉尘污染、提取率高、提取时间短，是一种高效提取龙胆多糖的方法。
Abstract:
The method of homogenate extraction technology of Gentiana scabra Bunge polysaccharides in Gentiana scabra Bunge was researched.To determine the best technical conditions and provides the evidence and reference for Gentiana scabra Bunge polysaccharides extraction from the medicinal material or preparation.The most important influence of homogenate extraction time,extraction times,the ratio of sample to solution were set as index for orthogonal test，so as to acquire the proper extraction technical conditions of Gentiana scabra Bunge polysaccharides.The results showed the optimum condition of extraction of parameters were homogenate extracting duration for 9 min with extracted 3 times at a ratio of solid-iquid of 1∶30( g∶mL).By using the optimized parameters，the extraction yield of Gentiana scabra Bunge polysaccharides was 12.59％.It may shorten the extraction time and high yield，which was an efficient method for extracting polysaccharides．It could be used for large scale industrialized production and provides the evidence and reference for Gentiana scabra Bunge polysaccharides extraction from the medicinal material or preparation.

参考文献/References:

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　WANG Chen-yu,LIU Xin,ZHANG Jing,et al.Study on Extraction of Water solubility Polysaccharide from Gentiana scabra Bunge[J].Northern Horticulture,2013,37(09):155.
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备注/Memo

第一作者简介：史伟国（1969-），男，博士，副教授，研究方向为植物药研究与开发。E-mail：swg19701210@163.com。
基金项目：黑龙江省卫生厅科研资助项目（2010-476）；黑龙江省卫生厅科研资助项目（2010-535）；林业公益性行业科研专项资助项目（201004040-02）；黑龙江省中医药管理局科研资助项目（ZHY10-Z112）;佳木斯大学大学生科技创新资助项目（Dz2011-017）。
收稿日期：2012-02-20

更新日期/Last Update: 2014-09-03