DU Gang,LAI Tian-chao,YANG Hai-ying.Study on the Tissue Culture of Dendrobium aphyllum（Roxb.) C.E.C.Fisch[J].Northern Horticulture,2012,36(08):140-141.
兜唇石斛的组织培养研究
- Title:
- Study on the Tissue Culture of Dendrobium aphyllum（Roxb.) C.E.C.Fisch
- 文章编号:
- 1001-0009（2012）08-0140-02
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以兜唇石斛的成熟蒴果作为外植体,比较不同的培养基、激素等因素对种子发芽及试管苗生根的影响。结果表明：以成熟蒴果为外植体能够诱导成苗，种子萌芽的最适培养基为：MS +NAA 0.5 mg/L+6-BA 2.0 mg/L+GA 1.0 mg/L+香蕉汁150 g/L+蔗糖 20 g/L+琼脂 8 g/L+活性炭 2 g/L,适宜的生根培养基为:1/4MS+NAA 2.0 mg/L+6-BA 0.5 mg/L+香蕉汁150 g/L+蔗糖 20 g/L+琼脂 8 g/L+活性炭 2 g/L。
- Abstract:
- Taking mature capsule of Dendrobium aphyllum（Roxb.) C.E.C.Fisch as explant,the effect of different medium and phytohormones on seeds germination and root induction of seedlings of Dendrobium aphyllum（Roxb.) C.E.C.Fisch.were studied.The results showed that seedlings could be induced from seed.The most appropriate medium for seedling inducing was MS+NAA 0.5 mg/L+6BA 2.0 mg/L+GA 1.0 mg/L+banana juice 150 g/L+sugar 20 g/L+agar 8 g/L+activated carbon 2 g/L.The most appropriate rooting medium was 1/4MS+NAA 2.0 mg/L+6-BA 0.5 mg/L+banana juice 150 g/L+sugar 20 g/L+agar 8 g/L+activated carbon 2 g/L.
参考文献/References:
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备注/Memo
第一作者简介：杜刚（1973-）,男,硕士,副教授,现主要从事植物组织培养及天然产物生物催化研究工作。E-mail:dugang2006@yahoo.com.cn。