PAN Xue-wu,DONG Yan-ling,HAN Xiao-hong.Study on the Expression System of Hairy Roots of Potential Recombinant Pharmaceutical Proteins[J].Northern Horticulture,2012,36(07):196-198.
极具潜力的重组药用蛋白的毛状根表达系统研究
- Title:
- Study on the Expression System of Hairy Roots of Potential Recombinant Pharmaceutical Proteins
- 文章编号:
- 1001-0009(2012)07-0196-03
- 分类号:
- S 567
- 文献标志码:
- A
- 摘要:
- 对植物表达系统产生重组药用蛋白进行了简单的介绍,重点论述了利用发根农杆菌诱导的毛状根培养系统表达重组药用蛋白的研究及应用情况。
- Abstract:
- Plant expression system for the production of recombinant pharmaceutical proteins was introduced,the focal point of which was the production of recombinant pharmaceutical proteins expressed by hairy root cultures induced by Agrobacterium rhizogenes and the application of this expression system
参考文献/References:
[1]Obembe O O,Popoola J O,Leelavathi S,et al.Advances in plant molecular farming [J].Biotechnology Advances,2011,29:210-222.
[2]Hiatt A,Cafferkey R,Bowdish K.Production of antibodies in transgenic plants [J].Nature,1989,342:76-78.
[3]Paul M,Ma J K.Plant-made pharmaceuticals:Leading products and production platforms [J].Biotechnology and Applied Biochemistry,2011,58:58-67.
[4]Zhou M L,Zhu X M,Shao J R,et al.Production and metabolic engineering of bioactive substances in plant hairy root culture [J].Appl Microbiol Biotechnol,2011,90:1229-1239.
[5]Desai P N,Shrivastava N,Padh H.Production of heterologous proteins in plants:Strategies for optimal expression [J].Biotechnology Advances,2010,28:427-435.
[6]Xu J F,Ge X M,Dolan M C.Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures[J].Biotechnology Advances,2011,29:278-299.
[7]Chandra S,Chandra R.Engineering secondary metabolite production in hairy roots [J].Phytochemistry Reviews,2011,10:371-395.
[8]Wongsamuth R,Doran P M.Production of monoclonal antibodies by tobacco hairy roots[J].Biotechnology and Bioengineering,1997,54:401-415.
[9]Sharp J M,Doran P M.Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions [J].Biotechnol Bioprocess Eng,1999(4):253-258.
[10]Sharp J M,Doran P M.Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures[J].Biotechnology Progress,2001,17:979-992.
[11]Martínez C,Petruccelli S,Giulietti A M,et al.Expression of the antibody 14D9 in Nicotiana tabacum hairy roots [J].Electronic Journal of Biotechnology,2005(8):170-176.
[12]Medina-Bolivar F,Wright R,Funk V,et al.A non-toxic lectin for antigen delivery of plant-based mucosal vaccines[J].Vaccine,2003,21:997-1005.
[13]Bhattacharyya R,Bhattacharya B.Development of a potent in vitro source of Phylanthus amarus roots with pronounced activity against surface antigen of the hepatitis B virus[J].In Vitro Cellular & Developmental Biology-Plant,2004,40:504-508.
[14]Sunil Kumar G B,Ganapathi T R,Srinivas L,et al.Expression of hepatitis B surface antigen in potato hairy roots[J].Plant Science,2006,170:918-925.
[15]Guzman G D,Walmsley A M,Webster D E,et al.Hairy roots cultures from different Solanaceous species have varying capacities to produce E.coli B-subunit heat-labile toxin antigen [M].Biotechnology Letters,2012.
[16]Woods R R,Geyer B C,Mor T S.Hairy-root organ cultures for the production of human acetylcholinesterase[J].BMC Biotechnology,2008,23:95-102.
[17]Liu J,Dolan M C,Reidy M,et al.Expression of bioactive single-chain murine IL-12 in transgenic plants[J].Journal of Interferon & Cytokine Research,2008,28:381-392.
[18]Liu C,Towler M J,Medrano G,et al.Production of mouse interleukin-12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor [J].Biotechnology and Bioengineering,2009,102:1074-1086.
[19]Parsons J,Wirth S,Dominguez M,et al.Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum:effect of tissue differentiation and sodium nitroprusside addition[J].International Journal of Biotechnology & Biochemistry,2010(6):131-138.
[20]范伟全,包晓群,韩树海,等.重组人胸腺素α1基因在黄芪毛状根中的表达 [J].中国老年学杂志,2010,30:1393-1395.
[21]Kim S R,Sim J S,Ajjappala H,et al.Expression and large-scale production of the biochemically active human tissue-plasminogen activator in hairy roots of Oriental melon (Cucumis melo)[J].Journal of Bioscience and Bioengineering,2012,113(1):106-111.
[22]Hunter P.A new era for plant biotechnology[J].EMBO reports,2011,12:504-507.
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备注/Memo
第一作者简介:潘学武(1975-),男,湖北恩施人,博士,讲师,现主要从事植物细胞工程等研究工作。责任作者:董妍玲(1977-),女,山东德州人,博士,副教授,现主要从事基因工程的教学与科研工作。基金项目:湖北省教育厅科学研究资助项目(B20114606);武汉市教育局科学研究资助项目(2010084)。
收稿日期:2012-02-01