[1]杨艳丽,刘兴菊,刘桂林,等.双抗虫基因转化欧美杨107的研究[J].北方园艺,2012,36(07):120-122.
　YANG Yan-li,LIU Xing-ju,LIU Gui-lin,et al.Study on Two Insect-resistant Gene Transformation of Poplar 107[J].Northern Horticulture,2012,36(07):120-122.
点击复制

双抗虫基因转化欧美杨107的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年07期 页码: 120-122 栏目: 出版日期: 2012-07-16
Title:
Study on Two Insect-resistant Gene Transformation of Poplar 107
文章编号:
1001-0009（2012）07-0120-03
作者:
杨艳丽; 刘兴菊; 刘桂林; 周宁; 付晓鹏; 梁海永
河北农业大学，河北省林木种质资源与森林保护重点实验室，河北 保定 071000
Author(s):
YANG Yan-li; LIU Xing-ju; LIU Gui-lin; ZHOU Ning; FU Xiao-peng; YANG Xu; LIANG Hai-yong
Agricultural University of Hebei,Hebei Key Laboratory for Tree Genetic Resources and Forest Protection，Baoding,Hebei 071000
关键词:
欧美杨107; 农杆菌; 遗传转化; 再生体系; 检测
Keywords:
Poplar 107; Agrobacterium tumefaciens; genetic transformation; regeneration system; detection
分类号:
S 792.11
文献标志码:
A
摘要:
以欧美杨107为试材,采用农杆介导法进行双抗虫基因(Bt Cry1Ac基因+API慈姑蛋白酶抑制剂基因)的遗传转化研究，探讨了影响欧美杨107的遗传转化的不同因素,初步建立了欧美杨107品种高效组织培养再生体系及遗传转化体系。结果表明：经PCR检测和虫试效果,证明了目的基因已经整合到欧美杨107的基因组中,从转基因株系中筛选出生长发育正常并抗虫能力强的优良株系。
Abstract:
Taking Poplar 107 as material,and two kinds of insect genes (BtCry1Ac genes and API genes) genetic transformation study was mediated by the agrobacterium,the impact factors to Poplar 107 during the genetic transformation were discussed,and the more efficient tissue culture regeneration and genetic transformation system for Poplar 107 were established.The results showed that after the PCR detection and worm test results,it proved that the aim gene had been integrated into the Popar 107 genome,and the target was to select the top-quality plants with normal growth and strongly construct insect ability from the transgenic plants.

参考文献/References:

[1]李荣森.微生物防治害虫[M].北京:科学出版社,1983.

[2]Barton K A，Whitely H R，Ying N S.Bacillus thuringiensisδ-endotoxin expressed in transgenic Nicotiana tabacum providesresistence to lepidepteran insects [J].Plant Phsiol，1987，85：1107-1109.
[3]田颖川,李太元,莽克强,等.抗虫转基因欧洲黑杨的培育[J].生物工程学报,1993,9(4):291-297.
[4]郝贵霞,朱祯,朱之悌.转CpTI基因毛白杨的获得[J].林业科学,2000,36(1):116-119.
[5]饶红宇,伍宁丰,陈英,等.杨树NL-80106转Bt基因植株的获得及抗虫性[J].植物资源与环境学报,2000,9(2):1-5.
[6]田颖川,郑均宝,虞红梅,等.转双抗虫基因杂种741毛白杨的研究[J].植物学报,2000,42(3):263-268.
[7]郑均宝,田颖川,高宝嘉,等.转双抗虫基因741毛白杨的选择及抗虫性[J].林业科学,2000,36(2):13-19.
[8]Horsch R B,Fry J E,Hofiman N L,et al.A simple and general method of transferring genes intoplants [J].Science,1985,227:1229-1231.
[9]Edwards K,Johnstone C,Thompson C.A simple and rapid method for the preparation of plant genomic DNA for PCR analysis [J].Nucleic Acids Research,1991,19(6):1349.(该文作者还有杨旭,工作单位同第一作者。)

相似文献/References:

[1]刘莉莉,李昌禹.毛状根发展现状研究[J].北方园艺,2014,38(24):178.
　LIU Li-li,LI Chang-yu.Development Status of the Hairy Root[J].Northern Horticulture,2014,38(07):178.
[2]高佩,尹锐,林彦萍,等.农杆菌介导的番茄遗传转化研究进展[J].北方园艺,2016,40(14):192.[doi:10.11937/bfyy.201614048]
　GAO Pei,YIN Rui,LIN Yanping,et al.Research Progresses of Agrobacterium-mediated Transformation on Tomato[J].Northern Horticulture,2016,40(07):192.[doi:10.11937/bfyy.201614048]
[3]梁雪莲,郑奕雄,庄东红,等.花生幼苗不同切断及其愈伤组织的农杆菌介导转化[J].北方园艺,2007,31(04):0.[doi:10.11937/bfyy.200704085]
　LIANG Xue-lianI,ZHEN Yi-xiong,ZHUANG Dong,et al.Peanut Genetic Transfomtion by Agrobacterium Tumerficiences[J].Northern Horticulture,2007,31(07):0.[doi:10.11937/bfyy.200704085]
[4]王栋鑫,彭棣,张爽.农杆菌介导木本植物遗传转化的研究进展[J].北方园艺,2018,42(02):181.[doi:10.11937/bfyy.20173329]
　WANG Dongxin,PENG Di,ZHANG Shuang.Advances in Agrobacterium-mediated Transformation of Woody Plants[J].Northern Horticulture,2018,42(07):181.[doi:10.11937/bfyy.20173329]
[5]王英哲,王一楠,任伟,等.Bar+CP4EPSPS基因转化紫花苜蓿的研究及抗性鉴定[J].北方园艺,2019,43(18):32.[doi:10.11937/bfyy.20184504]
　WANG Yingzhe,WANG Yinan,REN Wei,et al.Transformation of Alfalfa With Bar+CP4EPSPS Genes and Resistance Indentification[J].Northern Horticulture,2019,43(07):32.[doi:10.11937/bfyy.20184504]
[6]王静,唐静,崔悦婷,等.农杆菌介导的黑果枸杞遗传转化体系的建立[J].北方园艺,2020,44(01):104.[doi:10.11937/bfyy.20191730]
　WANG Jing,TANG Jing,CUI Yueting,et al.Establishment of Agrobacterium-mediated Genetic Transformation System in Lycium ruthenicium Murr.[J].Northern Horticulture,2020,44(07):104.[doi:10.11937/bfyy.20191730]

备注/Memo

第一作者简介：杨艳丽(1985-),女,在读硕士,研究方向为园林植物栽培生理。E-mail：yyl_2004@163.com。

责任作者：梁海永(1973-)，男，副教授，研究方向为林业生物技术。E-mail：lianghy@hebau.edu.cn。
基金项目：国家高技术研究发展计划(“863”计划)资助项目(2011AA100201)。
收稿日期：2012-01-10

更新日期/Last Update: 2014-09-03