SONG Li-li,SI Liang,YU Dian-si,et al.Study on Tissue Culture and Micropropagation of Lilium asiatic Hybrids Prato[J].Northern Horticulture,2012,36(06):122-124.
亚洲百合“普瑞头”叶片组织培养技术研究
- Title:
- Study on Tissue Culture and Micropropagation of Lilium asiatic Hybrids Prato
- 文章编号:
- 1001-0009（2012）06-0122-03
- Keywords:
- Lilium asiatic Hybrids ‘Prato’; tissue culture; callus; explant
- 分类号:
- S 682.2+9
- 文献标志码:
- A
- 摘要:
- 以亚洲百合普瑞头组培苗的叶片为外植体，对其组织培养技术进行了研究。结果表明:不同激素组合对愈伤组织的诱导和分化效果差异显著，诱导叶片愈伤组织的最适培养基为MS+0.5 mg/L 6-BA+0.5 mg/L NAA+0.2 mg/L KT+0.1 mg/L 2,4-D，诱导率为73.3%；愈伤组织分化的最佳培养基为MS+0.1 mg/L TDZ+0.5 mg/L NAA，生根效果最好的培养基为1/2MS+0.5 mg/L NAA。
- Abstract:
- The leaves of ‘Prato’ were selected as explants，the tissue culture of Lilium asiatic Hybrids Prato were studied.The results showed that the optimal medium for leaf induction was MS+0.5 mg/L 6-BA+0.5 mg/L NAA+0.2 mg/L KT+0.1 mg/L 2,4-D，the induction rate was 73.3%;MS+0.1 mg/L TDZ+0.5 mg/L NAA was the optimal medium for the callus differentiation；the 1/2MS+0.5 mg/L NAA was successful for rapid rooting.
参考文献/References:
[1]周蕴薇,刘艳萍，岳莉然，等.亚洲百合‘普瑞头’的组织培养及休眠小鳞茎获得的研究[J].北方园艺,2011(4):146-148.
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备注/Memo
第一作者简介：宋丽莉（1987)，女，在读硕士，研究方向为植物基因工程