HU Hong-sha,TANG Lin.A Primary Study on Tissue Culture of Camelia sinensis cv.Chongqingpipacha[J].Northern Horticulture,2012,36(05):122-124.
崇庆枇杷茶组织培养初步研究
- Title:
- A Primary Study on Tissue Culture of Camelia sinensis cv.Chongqingpipacha
- 文章编号:
- 1001-0009（2012）05-0122-03
- 分类号:
- S 571.1
- 文献标志码:
- A
- 摘要:
- 以崇庆枇杷茶的带芽茎段为试材，对外植体的消毒、愈伤的诱导、芽的启动和增殖进行了初步研究。结果表明：以培养室扦插苗的带芽茎段为材料，用多菌灵处理20 min，0.1%升汞消毒10 min后再用0.05%升汞消毒3 min效果最好，其存活率可达88.5%；适合愈伤诱导的培养基为MS+1 mg/L BA+0.5 mg/L NAA，愈伤颜色鲜绿，质地较硬，转入MS+（1、2 mg/L）TDZ中愈伤表面变为颗粒状；有利于芽启动的培养基为：MS+2 mg/L BA+0.05 mg/L IAA+0.5 mg/L GA3，有利于芽增殖的培养基为MS+1 mg/L TDZ+1 mg/L GA3，2种培养基交叉培养有利于芽的增殖。
- Abstract:
- Using Camelia sinensis cv.Chongqingpipacha buds as materials，explants sterilization，callus induction，and proliferation culture of buds were studied.The results showed that using cutting seedling in culturing room as explants，the optimal sterilization time was about 3 minutes with 0.05% HgCl2after the 10 minutes with 0.1% HgCl2，and its survival rate was about 88.5%.The optimal induction of callus was MS+1 mg/L BA+0.5 mg/L NAA.The callus color was bright green，and its texture was harder，then its surface turn to granular when was transferred to MS+（1,2 mg/L）TDZ.The best medium for proliferation of buds was MS+2 mg/L BA+0.05 mg/L IAA+0.5 mg/L GA3and MS+1 mg/L TDZ+1 mg/L GA3.The alternate cultivation on the two medium was in favor of proliferation of buds.
参考文献/References:
[1]江济和,邹瑚.论四川大茶树资源[J].茶叶通报,1993(3):20-23.
[2]钟渭基.崇庆枇杷种[J].茶叶,1959(5):31-32.
[3]郭元超.我国栽培茶树的起源与演化[J].福建茶叶,1991(3):10-18.
[4]林郑和,陈常颂,陈荣冰.我国39个茶树品种的RAPD分析[J].分子遗传育种,2006(5):695-701.
[5]侯渝嘉,彭萍,钟渭基,等.四川茶树种质资源鉴定与评价[J].西南农业大学学报,1998(3):235-238.
[6]罗凡,王云,李春华,等.四川茶树品种研究现状与发展趋势[J].贵州科学,2008,26(2):52-57.
[7]魏芳华.茶树短穗扦插技术研究[J].茶叶通报,1989(2):21-23.
[8]董时富.生物统计学[M].北京:科学出版社,2002:172-181.
[9]张建华,毛平生,彭火辉.茶树的组培快繁技术初探[J].蚕桑茶叶通讯,2003(4):32-33.
[10]何芳兰,李毅,赵明,等.影响高山杜鹃试管苗玻璃化的几个因素研究[J].西北林学院学报,2008,23(1):104-107.
相似文献/References:
[1]刘艳伟,吴景贵.有机栽培基质的研究现状与展望[J].北方园艺,2011,35(10):0.[doi:10.11937/bfyy.2011010055]
LIU Yan-wei,WU Jing-gui.Review on Researches of Organic Substrate for Culture[J].Northern Horticulture,2011,35(05):0.[doi:10.11937/bfyy.2011010055]
[2]刘月,叶维雁,王樱潼,等.葡萄柚成年态茎段外植体的消毒与抗褐化[J].北方园艺,2017,41(07):131.[doi:10.11937/bfyy.201707027]
LIU Yue,YE Weiyan,WANG Yingtong,et al.Disinfection and Browning Prevention for Mature Explant of Grapefruit[J].Northern Horticulture,2017,41(05):131.[doi:10.11937/bfyy.201707027]
[3]钟灿,劳嘉,金剑,等.基于激素及其配比探讨多花黄精种子组培体系的建立[J].北方园艺,2020,44(13):118.[doi:10.11937/bfyy.20194082]
ZHONG Can,LAO Jia,JIN Jian,et al.Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua[J].Northern Horticulture,2020,44(05):118.[doi:10.11937/bfyy.20194082]
备注/Memo
第一作者简介：胡洪沙（1984-），女，在读硕士，研究方向为风景园林。E-mail:huhongsha9@126.com。
责任作者：唐琳(1966-)，女，博士，副教授，硕士生导师，研究方向为植物学。E-mail: tangl66@sina.com。
收稿日期：2011-12-14