ZHANG Ying,MA Li-yuan,ZHANG Gui-xue,et al.In vitro Culture of Populus × euramericana cv.I-45[J].Northern Horticulture,2012,36(05):119-121.
I-45杨的离体培养
- Title:
- In vitro Culture of Populus × euramericana cv.I-45
- 文章编号:
- 1001-0009(2012)05-0119-03
- 分类号:
- S 792.11
- 文献标志码:
- A
- 摘要:
- 以I-45杨带腋芽的茎段为外植体,研究其离体培养中的最佳培养基和激素配比,建立I-45杨再生系统。结果表明:最佳诱导培养基为MS+KT 0.5 mg/L+NAA 0.02 mg/L,诱导率为82.2%;最佳继代培养基为MS+KT 1.0 mg/L+NAA 0.02 mg/L;诱导生根的培养基为1/2MS+IBA 0.05 mg/L,生根率达86.7%。
- Abstract:
- The suitable medium and plant growth regulator of plants were investigated by using popular axillary buds of nodal segments as explants.The results showed that MS medium supplemented with KT 0.5 mg/L and NAA 0.02 mg/L could induce shoots and the induction rate was 82.2%.Subcultured to MS medium added with KT 1.0 mg/L and NAA 0.02 mg/L could promote shoot growth.The rooting medium was 1/2MS medium in addition to IBA 0.05 mg/L,with the rooting rate of 86.7%.
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备注/Memo
第一作者简介:张瑛(1985-),女,在读硕士,现主要从事林木生物技术研究工作。
责任作者:崔建国(1969-),男,博士,教授,现主要从事林木生物技术和林木遗传改良研究工作。E-mail:cjgsau@yahoo.com.cn。
基金项目:沈阳市科学技术计划资助项目(F10-137-9-00)。
收稿日期:2011-11-29