ZHU Jian-guo,HAO Wen-fang,ZHAO Jie,et al.The Effects of Enhance UV-B Radiation on the Content Variation of UV-absorbing Compouds and Adjusting Osmotic Substances of Lespedeza davurica[J].Northern Horticulture,2012,36(05):13-17.
增强UV-B辐射对达乌里胡枝子紫外吸收物质和渗透调节物质含量的影响
- Title:
- The Effects of Enhance UV-B Radiation on the Content Variation of UV-absorbing Compouds and Adjusting Osmotic Substances of Lespedeza davurica
- 文章编号:
- 1001-0009(2012)05-0013-05
- Keywords:
- Lespedeza davurica; enhanced UV-B radiation; UV-absorbing compound; adjusting osmotic substances
- 分类号:
- Q 945
- 文献标志码:
- A
- 摘要:
- 以盆栽达乌里胡枝子为试材，研究在0（CK）、1.944（T-1）和3.002 kJ?m-2?d-1(T-2)3种UV-B辐射强度下，达乌里胡枝子叶片内紫外吸收物质和渗透调节物质含量的动态响应。结果表明：增强UV-B辐射下达乌里胡枝子叶片内类黄酮、总酚、花青素含量以及苯丙氨酸转氨酶活性均呈现先增加后降低的趋势，且大部分情况显著（P<0.05）高于对照组。不同剂量的UV-B辐射所引起的效应也略有不同，3.002 kJ?m-2?d-1强度处理紫外吸收物质（花青素除外）和苯丙氨酸转氨酶活性在紫外辐射前期显著(P<0.05)高于1.944 kJ?m-2?d-1强度处理。可溶性蛋白含量在UV-B辐射下大幅度下降，最终极显著（P<0.01）低于CK，且随着UV-B辐射强度的增加而降低。游离脯氨酸含量只有在1.944 kJ?m-2?d-1处理第6天时显著（P<0.05）高于对照组，其余情况下均未显著高于对照组。表明增强UV-B辐射主要促进达乌里胡枝子叶片内紫外吸收物质含量的增加，但是抑制了渗透调节物质的合成，尤其是抑制可溶性蛋白。
- Abstract:
- Taking pot cultivation Lespedeza davurica(Laxm.) Schindl as test material,the effects of enhanced UV-B radiations（0，1.944，3.002 kJ?m-2?d-1）on the content variation of UV-absorbing compounds and adjusting osmotic substances in the leaves were studied.The results showed that under the enhanced UV-B radiation，the contents of flavonoid，total hydroxybenzene，anthocyanin and the activity of phenylalanine ammonia-lyase increased at first and then decreased，and mostly they were significantly higher than that of the control(P<0.05).Also due to different doses of UV-B radiation，the effects were slightly different.The increase extent(in the early stage of UV-B radiation) of the content of UV-absorbing compounds(exceptfor anthocyanins)and the activity of phenylalanine ammonia-lyase under 3.002 kJ?m-2?d-1UV-B radiation are significantly larger than that of 1.944 kJ?m-2?d-1UV-B radiation(P<0.05).The content of soluble protein decreased significantly under the enhanced UV-B radiation(P<0.01)，and decreased with the increasing of UV-B radiation.Only on the sixth day and under 1.944 kJ?m-2?d-1UV-B radiation the content of free proline was significantly higher than that of the control(P<0.05)，which was not found in the the rest cases.These results implied that the enhanced UV-B radiation induced the synthesis of the UV-absorbing compounds in the leaves of Lespedeza davurica，but inhibited the synthesis of the adjusting osmotic substances especially soluble protein.
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备注/Memo
第一作者简介：朱建国（1987-），男，山东济宁人，在读硕士，研究方向为植物逆境生理。E-mail：zjgjx0537@163.com。
责任作者：郝文芳（1968-），女，陕西西乡人，博士，副教授，现主要从事植物生理生态学和恢复生态学研究工作。E-mail：haowenfang2002@tom.com。
基金项目：中国科学院“西部之光”人才培养资助项目（2008DF02）。
收稿日期：2011-12-28