[1]汪福源,唐宁,宋东杰.噻重氮苯基脲对贯叶金丝桃胚性愈伤诱导和分化的影响[J].北方园艺,2012,36(03):163-165.
　WANG Fu-yuan,TANG Ning,SONG Dong-jie.Effects of Different Concentrations of TDZ on Embroyogenic Callus Inductionand Differentiation of Hypericum perforatum L.[J].Northern Horticulture,2012,36(03):163-165.
点击复制

噻重氮苯基脲对贯叶金丝桃胚性愈伤诱导和分化的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年03期 页码: 163-165 栏目: 出版日期: 2012-03-16
Title:
Effects of Different Concentrations of TDZ on Embroyogenic Callus Inductionand Differentiation of Hypericum perforatum L.
文章编号:
1001-0009(2012)03-0163-03
作者:
汪福源1; 唐宁2 ; 宋东杰2
1.中国药科大学 高等职业技术学院，江苏 南京，211198；
2.南京晓庄学院 生物化工与环境工程学院，江苏 南京，211171
Author(s):
WANG Fu-yuan1; TANG Ning2; SONG Dong-jie2
1.School of Higher Vocational and Technical，China Pharmaceutical University，Nanjing，Jiangsu 211198；
2.School of Biochemistry and Environmental Engineering，Nanjing Xiaozhuang College，Nanjing，Jiangsu 211171
关键词:
贯叶金丝桃; 噻重氮苯基脲（TDZ）; 愈伤组织; 不定芽
Keywords:
Hypericum perforatum L.; TDZ; callus; adventitious bud
分类号:
S 567.23+9
文献标志码:
A
摘要:
系统研究了不同浓度噻重氮苯基脲(TDZ)对贯叶金丝桃胚性愈伤的诱导和植株再生的影响。结果表明：诱导胚性愈伤的最适外植体为叶柄，诱导率可达100%。在诱导胚性愈伤和分化的最适培养基MS+0.05 mg/L TDZ+0.2 mg/L NAA上，不定芽分化率为55%。第1代培养基上，愈伤增殖迅速，绿色的胚性细胞团数目从原来的单个生长提高为平均5.6个。经2、3代培养，愈伤停止生长，胚性细胞团数量逐渐减少。
Abstract:
The embryogenic callus induction and shoot regeneration of Hypericum perforatum L.were studied systematically through different concentrations of TDZ.The results indicated that the most suitable part for inducing embryogenic callus was the petiole.The inducing rate reached as high as 100%.The optimized medium for inducing embryogenic callus and plant regeneration was MS+0.05 mg/L TDZ+0.2 mg/L NAA.The adventitious buds differentiating rate was 55%.The size of callus can increase greatly in the first subculture on the optimized inducing medium and the average number of green-coloured masses of embryogenic cells on each callus was 5.6.On the second and third subculture，callus stopped growing further and the number of masses of embryogenic cells decreased gradually.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2010.

[2]李艳,曹学丽,付鹏,等.贯叶金丝桃活性成分及其分离纯化与检测方法的研究进展[J].药学进展,2007,32(1):15-20.
[3]胡倩,刘晓蕙,王林泉,等.贯叶金丝桃的药理作用及临床应用进展[J].中医研究,2006,19(7):56-58.
[4]任如意,王书臻,司徒琳莉,等.噻重氮苯基脲对文冠果愈伤组织诱导与分化的影响[J].北方园艺,2011(6):127-129.
[5]魏岳荣,杨护,黄秉智,等.Picloram,ABA和TDZ对香蕉体细胞胚胎发生的影响[J].园艺学报,2007,34(1):81-86.
[6]王亦菲,陆瑞菊,周润梅,等.贯叶连翘无菌苗茎尖诱导变异体研究[J].中国农学通报,2007,23(6):157-159.
[7]张娜.贯叶连翘植株再生体系的建立及Hyp基因表达的初步研究[D].北京:中国农业大学,2005:23-28.[8]Charchoglyana,Abrahamyana，Fujii I,et al.Differential accumulation of hyperforin and secohyperforin in Hypericum perforatum tissuecultures[J].Phytochemistry,2007,68(21):2670-2677.
[9]裘珍飞,曾炳山,李湘阳,等.TDZ对巨尾桉(GL9)胚性愈伤组织诱导和再生的影响[J].林业科学研究,2009,22(5):740-743.

备注/Memo

第一作者简介：汪福源（1978-），男，博士，讲师，现主要从事中药生物技术研究工作。

收稿日期：2011-11-23

更新日期/Last Update: 2014-08-27