[1]龙月红,邢朝斌,梁能松,等.刺五加鲨烯合酶基因2 cDNA的克隆与蛋白结构分析[J].北方园艺,2012,36(02):117-120.
　LONG Yue-hong,XING Zhao-bin,LIANG Neng-song,et al.Cloning of the Squalene Synthase Gene 2 cDNA from Eleutherococcus senticosus and Analysis of Its Putative Protein Structure[J].Northern Horticulture,2012,36(02):117-120.
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刺五加鲨烯合酶基因2 cDNA的克隆与蛋白结构分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年02期 页码: 117-120 栏目: 出版日期: 2012-02-16
Title:
Cloning of the Squalene Synthase Gene 2 cDNA from Eleutherococcus senticosus and Analysis of Its Putative Protein Structure
文章编号:
1001-0009(2012)02-0117-04
作者:
龙月红; 邢朝斌; 梁能松; 何闪; 李宝财; 朱金丽
河北联合大学 生命科学学院,河北 唐山 063000
Author(s):
LONG Yue-hong; XING Zhao-bin; LIANG Neng-song; HE Shan; LI Bao-cai; ZHU Jin-li
College of Life Science,Hebei United University,Tangshan,Hebei 063000
关键词:
鲨烯合酶基因; 刺五加; RT-PCR; RACE
Keywords:
squalene synthase gene; Eleutherococcus senticosus;  RT-PCR; RACE
分类号:
S 75982
文献标志码:
A
摘要:
采用RT-PCR和RACE法克隆了刺五加鲨烯合酶基因2（squalene synthase gene 2，SS2） cDNA的全长序列( GenBank登录号: JN714465)。该序列全长1 463 bp，其中5’端非翻译区长10 bp，3’端非翻译区长208 bp，开放阅读框长1 245 bp，编码414个氨基酸。该蛋白具有1个Trans_IPPS_HH保守区域、2个鲨烯合酶和八氢番茄红素合成酶特异性识别区域及2个跨膜区。氨基酸序列比对结果表明，该蛋白与五加科其它植物SS的一致性均大于90%，与刺五加SS1的一致性为9179%。
Abstract:
Full-length of Esenticosus squalene synthase gene 2 cDNA (GenBank number: JN714465) was cloned by RT-PCRand RACE methods.Sequence analysis revealed a 1 463 bp cNDA containing 10 bp 5′UTR,208 bp 3′UTR and 1 245bp ORF encoding 414 amino acids.The protein had one Trans_IPPS_HH conserved domain,two specificity target region of phytoene synthase and two thransmembrane helixs.Amino acids comparison showed that the consistence of the protain and SS in other Araliaceae plants was more than 90%.The consistence wrth E.senticosus SS1 was 9179%.

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备注/Memo

第一作者简介：龙月红(1974-),女,助理实验师,现主要从事药用植物的研究工作。E-mail:xzbheuu@126.com。责任作者：邢朝斌（1975-）,男,硕士,副教授,现主要从事分子生药学及药用植物细胞工程研究工作。E-mail: xingzhaobin@yahoo.com.cn。

基金项目：国家自然科学基金资助项目(30701086)；河北省自然科学基金资助项目（C2009001252）。
收稿日期：2011-10-27

更新日期/Last Update: 2014-08-24