|Table of Contents|

Transformation of Verticillium dahliae From Eggplant Based on ATMT and Characterization of Transformants

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年11
Page:
98-106
Research Field:
Publishing date:

Info

Title:
Transformation of Verticillium dahliae From Eggplant Based on ATMT and Characterization of Transformants
Author(s):
HU Kaifeng1; ZHANG Yuanyuan2; JIA Ruifang2; YAO Xue1; ZHANG Xueyan1; QU Hongyun1
(1.Horticultural Branch,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150036; 2.Grassland Research Institute,Chinese Academy of Agricultural Sciences,Hohhot,Inner Mongolia 010010)
Keywords:
eggplant Verticillium wilt; Verticillium dahliae; ATMT; GFP
PACS:
S432.44
DOI:
10.11937/bfyy.20253615
Abstract:
Taking the highly pathogenic strain EP-3 of eggplant Verticillium wilt fungus as the test material,an Agrobacterium tumefaciens-mediated genetic transformation (ATMT) system was employed to systematically optimize key transformation parameters,including concentrations of A.tumefaciens and acetosyringone (AS),as well as co-culture temperature and time,to establish optimal transformation conditions.The genetic expression stability of the obtained GFP-labeled transformants was assessed by analyzing characteristics such as growth rate,sporulation capacity,crude toxin secretion,and in order to pathogenicity,to establish and optimize a genetic transformation system for eggplant Verticillium wilt fungus and obtain green fluorescent protein (GFP)-labeled strains,and in order to provide reference for subsequent studies on the infection and colonization process of this pathogen in eggplants and its pathogenic mechanisms.The results showed that the optimal transformation conditions were as follows,A.tumefaciens concentration at OD600=0.6,conidial concentration of 1×105 conidia·mL-1,acetosyringone (AS) concentration of 200 μmol·L-1,and co-culture at 25 ℃ for 48 hours.Through hygromycin resistance screening,PCR,and fluorescence microscopy,it was confirmed that the GFP gene had been successfully integrated into the genome of strain EP-3 and was stably expressed.Analysis of transformant characteristics indicated that the obtained transformants exhibited stable core biological traits,with no significant differences compared to the wild-type strain.These findings further demonstrate that an efficient genetic transformation system for eggplant Verticillium wilt fungus has been successfully established in this study.

References:

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Last Update: 2026-06-23