|Table of Contents|

Cloning of SmWRKY1 Transcription Factor Gene and Its Transformation in Arabidopsis thaliana

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年4
Page:
81-89
Research Field:
Publishing date:

Info

Title:
Cloning of SmWRKY1 Transcription Factor Gene and Its Transformation in Arabidopsis thaliana
Author(s):
LING Na1; YANG Zhaoxia1; YANG Yalan1; LIU Lian1; XING Shuoming1; MENG Fanrong2
(1.College of Architecture and Landscape,Shangqiu University,Shangqiu,Henan 476000；2.College of Life Science,Henan Agricultural University,Zhengzhou,Henan 450002)
Keywords:
Savila miltiorkriza; SmWRKY; gene cloning; genetic transformation
PACS:
Q 943.2
DOI:
10.11937/bfyy.20232986
Abstract:
Taking Salvia miltiorrhiza as the test material,total RNA and synthetic cDNA were extracted.The transcription factor SmWRKY1 gene sequence of Salvia miltiorrhiza was obtained by PCR amplification.The physicochemical properties of the gene encoding protein and amino acid multi sequence alignment were detected using bioinformatics software and online tools.An overexpression vector was constructed and transformed into Arabidopsis using floral infection method.The effect of SmWRKY1 gene transformation on the growth traits and physiological indicators of Arabidopsis was studied,in order to preliminarily explore the biological functions of SmWRKY1 transcription regulatory factors.The results showed that the SmWRKY1 gene had a total length of 1 041 bp and encodes 346 amino acids.BLAST analysis of the conserved region in the NCBI database showed a ‘WRKY’ DNA binding conserved domain and a C2H2 zinc finger structure.The molecular weight of the encoded protein was 37 310.4 Da，the Isoelectric point pI was 9.77，and the GRAVY was -0.571.ProtParam analysis showed that SmWRKY1 was a hydrophilic protein.Construction of overexpression vector pCAMBIA-SmWRKY1 and transfer to Arabidopsis using inflorescence infection method，11 positive seedlings with SmWRKY1 gene were identified.Transgenic plants could significantly increase leaf and rosette diameter，increased chlorophyll and soluble sugar content，and reduced MDA and proline content.Therefore，it was speculated that the SmWRKY1 factor of Salvia miltiorrhiza might be a negative regulatory factor related to plant basic defense，participating in plant abiotic stress response.

References:

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Last Update: 2024-03-15