|Table of Contents|

Effects of Bacterial Wilt on the Microbial Community Structure in Rhizosphere Soil and Root Endosphere of Greenhouse-grown Pepper

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年15
Page:
95-104
Research Field:
Publishing date:

Info

Title:
Effects of Bacterial Wilt on the Microbial Community Structure in Rhizosphere Soil and Root Endosphere of Greenhouse-grown Pepper
Author(s):
NIE Xiaoying1; LU Yacheng1; LI Yang2; HUANG Chunping1; QIN Guobing1; LU Fashi1
(1.Hezhou Branch,Guangxi Academy of Agricultural Sciences/Hezhou Academy of Agricultural Sciences,Hezhou,Guangxi 542899;2.Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)
Keywords:
greenhouse pepper; bacterial wilt; rhizosphere microbiome; root endosphere microbiome; Illumina miSeq
PACS:
S641.3
DOI:
10.11937/bfyy.20260471
Abstract:
Taking healthy and bacterial wilt-infected pepper plants in a facility greenhouse as test materials,Illumina MiSeq high-throughput sequencing was employed to investigate the effects of bacterial wilt on the microbial community structures in the rhizosphere soil and root endosphere of greenhouse pepper,in order to provide references for the prevention and control of bacterial wilt through the regulation of rhizosphere/root endosphere microbial communities.The results showed that compared with healthy plants,the alpha diversity (OTUs,Chao1,and Shannon indices) of bacterial communities in both the rhizosphere and root endosphere of diseased plants was significantly reduced (P<0.05).In diseased plants,the relative abundances of Proteobacteria and Ralstonia (the pathogen causing bacterial wilt) were significantly increased in both the rhizosphere and root endosphere,with the abundance of Ralstonia reaching as high as 99.12% in the root endosphere.Meanwhile,the abundances of beneficial bacterial genera such as Lysobacter and Agrobacterium in the rhizosphere soil,and Streptomyces in the root endosphere,were significantly decreased.Regarding fungi,the community evenness (Shannon index) in both the rhizosphere and root endosphere of diseased plants was significantly reduced.The abundance of Penicillium in the rhizosphere of diseased plants was significantly increased,while the abundances of Fusarium and Thanatephorus in the root endosphere were extremely significantly increased (P<0.01).Principal coordinate analysis (PCoA) revealed significant differences in the bacterial communities of the rhizosphere and root endosphere,as well as in the fungal communities of the rhizosphere,between healthy and diseased plants (P<0.05).The occurrence of pepper bacterial wilt was accompanied by drastic changes in the microbial community structures of both the rhizosphere and root endosphere,characterized by a significant decrease in bacterial diversity,substantial enrichment of the pathogen Ralstonia and potentially pathogenic fungi (e.g.,Fusarium),and a marked reduction in the abundances of various beneficial microorganisms.

References:

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Last Update: 2026-08-14