|Table of Contents|

Effects of Exogenous Nitric Oxide (NO) on the Growth and Physiological Characteristics of ‘Jingjia 301’ Watermelon Under Waterlogging Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年9
Page:
52-61
Research Field:
Publishing date:

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Title:
Effects of Exogenous Nitric Oxide (NO) on the Growth and Physiological Characteristics of ‘Jingjia 301’ Watermelon Under Waterlogging Stress
Author(s):
FENG Ruchao1; ZHENG Jiaqiu1; ZHANG Jie2; YOU Chun3; LIU Zhe1; MEI Yi1
(1.Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng,Jiangsu 224002;2.Research Center for Vegetables,Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops （North China）,Ministry of Agriculture and Rural Affairs/Beijing Vegetable Germplasm Improvement Lab,Beijing 100097; 3.Yancheng Institute of Vegetables,Yancheng,Jiangsu 224002)
Keywords:
‘Jingjia 301’ watermelon; waterlogging stress; NO; antioxidant enzyme; osmotic adjustment
PACS:
S651
DOI:
10.11937/bfyy.20253507
Abstract:
Taking the ‘Jingjia 301’ watermelon cultivar as the test material,the effects of different concentrations (0,0.05,0.10,0.20,0.30 mmol·L-1) of sodium nitroprusside (SNP) on the number of leaves,vine length,stem diameter,leaf antioxidant enzyme activity and osmotic adjustment substance content of watermelon plants under waterlogging stress were studied by using the pot-in-pot waterlogging method,combined with principal component analysis,in order to provide a reference for exploring the role of exogenous nitric oxide (NO) in alleviating the damage of waterlogging stress to watermelon plants.The results showed that waterlogging stress had serious adverse effects on the growth of watermelon plants,slowing down the growth rate,causing stunted growth,yellowing,withering,and shedding of leaves.However,spraying 0.05 mmol·L-1 SNP could alleviate the damage of waterlogging stress to watermelon plants,delay the reduction of the number of leaves,vine length,and stem diameter growth rate,increase the activities of peroxidase (POD),superoxide dismutase (SOD),catalase (CAT),and proline (Pro) content in watermelon plants,and reduce the content of malondialdehyde (MDA) in plants;the results of principal component analysis showed that the waterlogging tolerance of watermelons treated with 0.05,0.10,0.20,0.30,and 0 mmol·L-1 SNPs decreased sequentially.In conclusion,it is demonstrated that a certain concentration of sodium nitroprusside treatment can regulate the activity of antioxidant enzymes and balance the content of osmotic adjustment substances in watermelon plants to alleviate the damage caused by waterlogging stress and enhance the waterlogging tolerance of watermelons.

References:

［1］刘文革,徐小利,潘秀清,等.黄河故道地区西瓜甜瓜产业分析和建议［J］.中国瓜菜,2022,35(8):1-11.［2］王娟娟,李莉,尚怀国.我国西瓜甜瓜产业现状与对策建议［J］.中国瓜菜,2020,33(5):69-73.［3］冯汝超,刘哲,沈峰,等.西瓜新品种京嘉301及其高效栽培技术［J］.蔬菜,2023(3):72-75.［4］陈传红,吴斌华,李荣同,等.淹水对西瓜幼苗SOD、POD活性和某些营养物质的影响［J］.江苏农业科学,2008,36(5):146-147,274.［5］李长根,马江黎,杨瑞平,等.生长调节剂对西瓜淹水胁迫的缓解效果［J］.农学学报,2022,12(10):30-34.［6］束秀玉,马江黎,李长根,等.8种不同药剂对西瓜涝害胁迫的恢复效果初探［J］.农学学报,2020,10(12):68-72.［7］李斯杨.中国洪涝灾害的成因类型以及防洪减灾应对方法［J］.中国新技术新产品,2011(1):253-254.［8］成伟.硫化氢参与植物细胞水涝低氧胁迫应答反应机制的研究［D］.兰州:兰州大学,2015.［9］SAIRAM R K,DHARMARK,CHINNUSAMYV,et al.Waterlogging-induced increase in sugar mobilization,fermentation,and related gene expression in the roots of mung bean (Vigna radiata)［J］.Journal of Plant Physiology,2009,166(6):602-616.［10］SASIDHARAN R,BAILEY-SERRES J,ASHIKARIM,et al.Community recommendations on terminology and procedures used in flooding and low oxygen stress research［J］.The New Phytologist,2017,214(4):1403-1407.［11］LEE S C,MUSTROPHA,SASIDHARANR,et al.Molecular characterization of the submergence response of the Arabidopsis thaliana ecotype Columbia［J］.The New Phytologist,2011,190(2):457-471.［12］陈杰,高青海,马横宇,等.西瓜砧木苗期耐涝性鉴定及指标选择［J］.北方园艺,2018(8):7-13.［13］王芳.大豆耐淹性鉴定及其形态解剖特征、遗传与QTL定位［D］.南京:南京农业大学,2007.［14］邓祥宜,李继伟,阳超男,等.淹水胁迫下小麦根通气组织形成的PCD特征及活性氧作用初探［J］.麦类作物学报,2009,29(5):832-838.［15］李林,刘登望,熊璟,等.花生生育早期耐涝性室内鉴定对大田期的意义［J］.作物学报,2008,34(3):477-485.［16］高青海,葛伟强,陈杰.淹水胁迫下不同砧木对嫁接西瓜产量和品质的影响［J］.分子植物育种,2019,17(24):8309-8314.［17］浦超群,李建军,蒋立辉.西瓜小拱棚早熟避雨栽培技术［J］.上海农业科技,2013(4):69.［18］戴照义,郭凤领,王运强,等.江汉平原西甜瓜避雨栽培技术［J］.长江蔬菜,2012(24):65-67.［19］肖光辉,梁志怀,贺爱国,等.南方多雨潮湿地区西瓜全程避雨高产栽培技术［J］.蔬菜,2012(7):24-27.［20］BRUCE T J A,MATTHES M C,NAPIER J A,et al.Stressful “memories” of plants:Evidence and possible mechanisms［J］.Plant Science,2007,173(6):603-608.［21］BAILEY-SERRES J,FUKAOT,GIBBS D J,et al.Making sense of low oxygen sensing［J］.Trends in Plant Science,2012,17(3):129-138.［22］杜宾.高温胁迫下NO信号途径调控辣椒AsA-GSH循环的机制［J］.北方园艺,2024(14):8-18.［23］张艳军,孔祥强,董合忠.一氧化氮对棉花淹水伤害的缓解作用及其生理机制［C］.郑州：中国农学会棉花分会2017年年会暨第九次会员代表大会,2017.［24］张健,刘美艳.外源一氧化氮提高玉米抗芽涝的研究［J］.玉米科学,2014,22(5):76-80.［25］张健,刘美艳.一氧化氮提高黄瓜幼苗耐涝性的探究［J］.中国瓜菜,2017,30(1):18-21.［26］廖向新.外源一氧化氮对垂丝海棠耐涝性的影响研究［D］.扬州:扬州大学,2020.［27］CHEN T S,YUAN F,SONG J,et al.Nitric oxide participates in waterlogging tolerance through enhanced adventitious root formation in the euhalophyte Suaeda salsa［J］.Functional Plant Biology,2016,43(3):244-253.［28］DA-SILVA C J,SHIMOIA E P,POSSO D A,et al.Nitrate nutrition increases foliar levels of nitric oxide and waterlogging tolerance in soybean［J］.Acta Physiologiae Plantarum,2021,43(8):116.［29］ZUBUKU S,ERGN N,〖AKI·〗LHAN E.Waterlogging and nitric oxide induce gene expression and increase antioxidant enzyme activity in wheat (Triticum aestivum L.)［J］.ActaBiologica Hungarica,2014,65(1):47-60.［30］HAMURCU M,KHAN M K,PANDEYA,et al.Nitric oxide regulates watermelon (Citrullus lanatus) responses to drought stress［J］.3 Biotech,2020,10(11):494.［31］HAKKI E E,HAMURCUM,GEZGINS,et al.Physiological responses of two contrasting watermelon genotypes exposed to drought and nitric oxide［J］.Journal of Biotechnology,2016,231:S25.［32］FARAG M,NAJEEBU,YANG JH,et al.Nitric oxide protects carbon assimilation process of watermelon from boron-induced oxidative injury［J］.Plant Physiology and Biochemistry,2017,111:166-173.［33］KHAN J,MALANGISHA G K,ALI A,et al.Nitric oxide alleviates lead toxicity by inhibiting lead translocation and regulating root growth in watermelon seedlings［J］.Horticulture,Environment,and Biotechnology,2021,62(5):701-714.［34］陈宇.一氧化氮提高苎麻耐涝性的生理与分子机制研究［D］.南宁:广西大学,2024.［35］杨利艳,杨小兰,朱满喜,等.干旱胁迫对藜麦种子萌发及幼苗生理特性的影响［J］.种子,2020,39(9):36-40.［36］郭春芳,罗玲娜,何水平,等.土壤水分胁迫下茶树部分渗透调节物质的变化［J］.中国农学通报,2015,31(28):126-131.［37］吴雅茹.旱柳对涝渍胁迫的生理响应及抗涝关键基因挖掘［D］.曲阜:曲阜师范大学,2024.［38］郭晨宁,李晶晶,杨灏,等.涝渍胁迫对地黄生理生化特性及活性成分的影响［J］.江苏农业科学,2025,53(11):118-126.［39］梁甜甜,张艳军,李燕,等.褪黑素缓解植物涝渍胁迫的生理和分子机制［J］.植物生理学报,2023,59(1):44-54.［40］曹文月,王宁,秦家乐,等.外源褪黑素对淹水胁迫下文冠果幼苗生长及生理特性的影响［J］.江苏农业科学,2025,53(1):209-217.［41］ZENG N B,YANG Z J,ZHANG Z F,et al.Comparative transcriptome combined with proteome analyses revealed key factors involved in alfalfa (Medicago sativa) response to waterlogging stress［J］.International Journal of Molecular Sciences,2019,20(6):1359.［42］朱敏,史振声,李凤海.玉米耐涝机理研究进展［J］.玉米科学,2015,23(1):122-127,133.［43］季艳林,赖慧灵,郑茹萍,等.植物涝渍胁迫应激机制研究进展［J］.生物技术进展,2016,6(1):1-5.［44］WANG B,LIU H F,LI C H,et al.Effects of nitric oxide on some physiological characteristics of maize seedlings under waterlogging［J］.African Journal of Agricultural Research,2011,6(19):4501-4504.［45］FAN H F,DU C X,DING L,et al.Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings［J］.Russian Journal of Plant Physiology,2014,61(3):366-373.［46］刘光亚,张艳军,孙学振,等.一氧化氮对植物淹水伤害的缓解作用及其机制［J］.分子植物育种,2019,17(22):7579-7587.［47］薛逸琳,王紫菡,程功,等.植物对淹水胁迫的响应及调节机制［J/OL］.分子植物育种,（2025-05-30）［2025-09-28］.https://link.cnki.net/urlid/46.1068.S.20250530.1152.004.［48］LU H Y,WANG M,LI W X,et al.Superior antioxidant capacity and auxin production promote seedling formation of rice seeds under submergence stress［J］.Agronomy,2023,13(1):171.［49］赵婷,李琴,潘学军,等.陆生植物对淹水胁迫的适应机制［J］.植物生理学报,2021,57(11):2091-2103.［50］黄晓玲,詹咪莎,王晓雨,等.不同牡丹品种在淹水胁迫下的生理响应［J］.北方园艺,2025(9):95-103.［51］余扣花,王红梅,刘凤琼,等.番茄砧木苗期对淹水胁迫响应及耐涝性评价［J］.中国蔬菜,2025(6):110-120.［52］王湘莹,魏溧姣,王晓明,等.淹水胁迫对紫薇苗生长及生理特性的影响［J］.北方园艺,2024(15):60-66.［53］张玉帅.外源一氧化氮对甜樱桃涝后恢复过程中生理生化特性影响的研究［D］.泰安:山东农业大学,2008.［54］徐洪雷.一氧化氮对黄瓜盐胁迫及低温胁迫缓解作用的研究［D］.哈尔滨:东北农业大学,2006.

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Last Update: 2026-05-19