|Table of Contents|

Authenticity Identification of Spathiphyllum kochii F1 Hybrids Using SSR Markers and Morphology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年6
Page:
18-27
Research Field:
Publishing date:

Info

Title:
Authenticity Identification of Spathiphyllum kochii F1 Hybrids Using SSR Markers and Morphology
Author(s):
LIN Huifeng12XU Kezheng12LI Lihong12LIN Fazhuang12XIE Chang12ZHOU Huiming12
(1.Institute of Flowers,Sanming Academy of Spathiphyllum kochii Sciences,Sanming,Fujian 365051; 2.Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization for Mountain Area,Sanming,Fujian 365051)
Keywords:
Spathiphyllum kochiimorphological identificationSSR markersspecies authentication
PACS:
S682
DOI:
10.11937/bfyy.20252797
Abstract:
Taking nine parental lines of Spathiphyllum kochii,eight hybrid F1 progenies,and a population of 39 hybrid F1 plants from the cross ‘Xingguang’בMeijiu’ as the test materials,polymorphic SSR marker analysis with morphological observation were combined to compare the efficiency of SSR markers and morphological methods in authenticating the hybridity of Spathiphyllum kochii F1 progenies,in order to establish an efficient and accurate early identification system to support Spathiphyllum kochii breeding.The results showed that eight pairs of SSR primers with paternal specific or complementary parental bands were selected from 16 polymorphic primers for hybrid identification.SSR amplification of the eight hybrid progenies all displayed paternal or biparental genotypes,achieving an authentication rate of 100%.Morphological observation clearly identified only six of the progenies (‘Qingmei’‘Xinghui’ ‘Weimei’‘Yangmeng 1’‘Yangmeng 2’ and ‘Weimo’) as true hybrids,while ‘Yushou’ and ‘Mengxiang’ could not be reliably distinguished based on morphology,giving a morphological identification efficiency of 75%.The efficiency of SSR markers was significantly higher than that of morphological methods,especially for true hybrids that did not exhibit obvious paternal traits.Further detection using the P14 marker on the 39 progenies from the ‘Xingguang’בMeijiu’ cross all produced paternal band patterns,with a true hybrid rate of 100%,reconfirming the high efficiency and reliability of SSR markers.In conclusion,SSR marker identification is accurate and efficient,and can serve as a key technique for early authenticity assessment of Anthurium hybrid progenies.

References:

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Last Update: 2026-04-01