|Table of Contents|

Determination of Low-temperature Half-lethal Temperature and Evaluation of Cold Resistance of Fifteen Brassica rapa L.Varieties

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年6
Page:
83-90
Research Field:
Publishing date:

Info

Title:
Determination of Low-temperature Half-lethal Temperature and Evaluation of Cold Resistance of Fifteen Brassica rapa L.Varieties
Author(s):
WEI Shuhua1; 2; WANG Ling1; 2; ZHANG Yue3; CUI Senyao1; 2; FAN Lihao1; 2; HAI Meirong1; 2
(1.College of Agronomy and Biotechnology，Yunnan Agricultural University，Kunming，Yunnan 650500 2.Nationa-Local Joint Engineering Research Center on Gemplasm Innovation & Utlization of Chinese Medicinal Materials in Southwest China，Kunming，Yunnan 650500 3.College of Plant Science & Technology,Huazhong Agricultural University，Wuhan，Hubei 430070)
Keywords:
Brassica rapa L.; low-temperature stress; semi-lethal temperature (LT50); low-temperature tolerance coefficient
PACS:
S631.3
DOI:
10.11937/bfyy.20252764
Abstract:
Taking 15 local turnip (Brassica rapa L.) germplasm resources collected by the research team as experimental materials,artificial low-temperature simulation was conducted to investigate the responses of relative electrical conductivity (REC) of leaves,as well as growth and physiological indexes of turnip seedlings at four-leaf-and-one-heart stage under low temperature treatments of 4 ℃,0 ℃,-2 ℃,-4 ℃,-6 ℃ and -8 ℃.The REC data under each temperature treatment were fitted by Logistic equation to calculate the low-temperature semi-lethal temperature (LT50).Combined with principal component analysis (PCA) and membership function analysis to comprehensively evaluate the cold tolerance potential of the 15 materials,in order to provide reference for cold-resistant breeding of turnips and their cultivation in high-altitude cold regions.The results showed that the low-temperature sublethal temperatures for the 15 materials were -6.88,-5.95,-5.37,-5.16,-4.64,-4.21,-3.93,-2.56,-2.48,-2.38,-2.25,-1.98,-1.13,-1.09 ℃ and -0.92 ℃.Among these，WJ1 exhibited the lowest low-temperature sublethal temperature，while WJ12 showed the highest.Through comprehensive evaluation，the 15 materials were classified into 2 cold-tolerant types，9 moderately cold-tolerant types，and 4 cold-sensitive types.Among these，2 cold-tolerant materials，WJ1 and WJ6，were selected.A preliminary quantitative regression equation predicting turnip seedling cold tolerance was obtained Y=-0.489+0.081X1+0.178X2+0.181X3.

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Last Update: 2026-04-01