|Table of Contents|

Effects of Exogenous Auxin and Cytokinin Application on the Healing Process of Oriental Melon Grafted Onto Pumpkin Under Low Night Temperature

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年2
Page:
38-43
Research Field:
Publishing date:

Info

Title:
Effects of Exogenous Auxin and Cytokinin Application on the Healing Process of Oriental Melon Grafted Onto Pumpkin Under Low Night Temperature
Author(s):
LIANG HuanLIU JiangfengZHU JuhongWANG DehuanGE MihongZHOU Mobing
(Institute of Crop,Wuhan Academy of Agricultural Sciences,Wuhan,Hubei 430345)
Keywords:
oriental melongrafting healingauxincytokininlow night temperature
PACS:
S 652
DOI:
10.11937/bfyy.20242647
Abstract:
Taking the thin skinned sweet melon ‘Meinong’ as the scion and the pumpkin variety ‘Deli No.2’ as the rootstock,20 different concentrations and types of auxin and cytokinin treatments were set at a temperature of 28 ℃/18 ℃ to observe the connection between the xylem and phloem during the grafting healing process.The effect of exogenous substance spraying on the grafting healing process of thin skinned sweet melon was explored,in order to provide reference for the efficient grafting and healing of melon.The results showed that spraying 0.5 mg·L-1 IAA or 10 mg·L-1 KT could promote the xylem connectivity.Compared with CK,at 5 days after grafting,the xylem connectivity of melon grafted seedlings treated with 0.5 mg·L-1 IAA and 10 mg·L-1 KT increased by 236.00% and 89.00%,respectively.Furthermore,0.5 mg·L-1 IAA and 10 mg·L-1 KT mixed with five ratio.The optimum hormone combination ratio was 1∶1,which significantly promoted the phloem and xylem connectivity of melon grafting.At 5 days after grafting,the phloem and xylem connectivity rates of melon grafted seedlings increased by 42.99% and 246.50%,respectively.In conclusion,the appropriate hormone and concentration can effectively accelerate the healing process.

References:

[1]LEE J M,KUBOTA C,TSAO S J,et al.Current status of vegetable grafting:Diffusion,grafting techniques,automation[J].Scientia Horticulturae,2010,127(2):93-105.[2]XU C,WU F,GUO J,et al.Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash[J].PeerJ,2022(10):e13980.[3]姜宇,王娟,吴春友,等.不同砧木嫁接对金冠王甜瓜生长及品质的影响[J].中国瓜菜,2024,37(3):80-86.[4]刘明,李梦竹,孙齐宇,等.四种嫁接方法对甜瓜嫁接苗生长的影响[J].北方园艺,2021(4):40-45.[5]LOUPIT G,BROCARD L,OLLAT N,et al.Grafting in plants:Recent discoveries and new applications[J].Journal of Experimental Botany,2023,74(8):2433-2447.[6]FENG M,AUGSTEIN F,KAREEM A,et al.Plant grafting:Molecular mechanisms and applications[J].Molecular Plant,2024,17(1):75-91.[7]NIE W,WEN D.Study on the applications and regulatory mechanisms of grafting on vegetables[J].Plants,2023(12):2822.[8]DEVI P,LUKAS S,MILES C.Advances in watermelon grafting to increase efficiency and automation[J].Horticulturae 2020(6):88.[9]QIU L,JIANG B,FANG J,et al.Analysis of transcriptome in hickory (Carya cathayensis),and uncover the dynamics in the hormonal signaling pathway during graft process[J].BMC Genomics,2016,17(1):935.[10]CHEN Z,ZHAO J,HU F,et al.Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile[J].Scientific Reports,2017,7(1):3954.[11]YIN H,YAN B,SUN J,et al.Graft-union development:A delicate process that involves cell-cell communication between scion and stock for local auxin accumulation[J].Journal of Experimental Botany,2012,63(11):4219-4232.[12]XU C,ZHANG Y,ZHAO M,et al.Transcriptomic analysis of melon/squash graft junction reveals molecular mechanisms potentially underlying the graft union development[J].PeerJ,2021(9):e12569.[13]CUI Q,XIE L,DONG C,et al.Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin[J].Plant Science,2021,304:110803.[14]汤雨凡,魏雪妮,侯银鹤,等.十一种甜瓜嫁接砧木苗期耐低温性比较[J].北方园艺,2022(24):17-23.[15]XU J,WEI X,XIONG M,et al.A method for simultaneously monitoring phloem and xylem reconnections in grafted watermelon seedlings[J].Scientia Horticulturae,2022,299:111058.[16]MELNYK C W,SCHUSTER C,LEYSER O,et al.A developmental framework for graft formation and vascular reconnection in Arabidopsis thaliana[J].Current Biology,2015,25(10):1306-1318.[17]KAWAGUCHI K,NOTAGUCHI M,OKAYASU K,et al.Plant hormone profiling of scion and rootstock incision sites and intra-and inter-family graft junctions in Nicotiana benthamiana[J].Plant Signaling & Behavior,2024,19(1):2331358.[18]YANG X,HU X,ZHANG M,et al.Effect of low night temperature on graft union formation in watermelon grafted onto bottle gourd rootstock[J].Scientia Horticulturae,2016,212:29-34.[19]XIE L,DONG C,SHANG Q.Gene co-expression network analysis reveals pathways associated with graft healing by asymmetric profiling in tomato[J].BMC Plant Biology,2019,19(1):373.[20]KSE C,GLERYZ M.Effects of auxins and cytokinins on graft union of grapevine(Vitis vinifera)[J].New Zealand Journal of Crop and Horticultural Science,2006,34(2):145-150.[21]张颖,刘义玲,汤雨凡,等.外源NAA对甜瓜嫁接愈合关键期保护性酶活性及相关基因表达的影响[J].中国瓜菜,2019,32(9):7-13.

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Last Update: 2025-02-08