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Strains of High Yield Polysaccharide on Auricularia auricula Selected by 60Coγray Mutagenesis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年2
Page:
8-13
Research Field:
Publishing date:

Info

Title:
Strains of High Yield Polysaccharide on Auricularia auricula Selected by 60Coγray Mutagenesis
Author(s):
LIU YanyanLIU JunjieLYU LitaoLI Hong
(Edible Fungi Institute,Liaoning Academy of Agricultural Seiences,Shenyang,Liaoning 110161)
Keywords:
60Coγraypolysaccharide of Auricularia auriculamutagenesis selection
PACS:
S 646.6
DOI:
10.11937/bfyy.20242642
Abstract:
‘Heifeng 2’ with high yield polysaccharide of Auricularia auricula that was previously screened out was selected as the starting strain.The mutagenesis conditions of Auriculus auricula after 60Coγray mutagenesis were studied through primary screening,rescreening,antagonism test,ISSR molecular marker verification and genetic stability test,in order to obtain Auriculus auricula with high yield polysaccharide.The results showed that the strain of Auricularia auricula with high yield polysaccharide was selected under the irradiation dose of 1 800 Gy and dose rate of 2.5 Gy·min-1.The results of antagonism test and ISSR molecular labeling test showed that the strain was mutagenic.Compared with the original strain,the polysaccharideyield of hypha was increased bynearly 30% by liquid culture.The polysaccharide yield of hypha was stable after 6 subcultures,indicated good genetic stability.

References:

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Last Update: 2025-02-08