|Table of Contents|

Isolation and Identification of Pathogen of Cucumber Corynespora Leaf Spot in the Suburb of Nanchang City

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年4
Page:
112-117
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogen of Cucumber Corynespora Leaf Spot in the Suburb of Nanchang City
Author(s):
ZHANG Jinyi; CHENG Shuyuan; WANG Quanxing; LIU Bing; JIANG Junxi; XIONG Guihong
(College of Agronomy，Jiangxi Agricultural University，Nanchang,Jiangxi 330045)
Keywords:
cucumber Corynespora leaf spot; pathogen identification; Corynespora cassiicola
PACS:
S 436.421.1
DOI:
10.11937/bfyy.20242613
Abstract:
The cucumber leaves with typical symptoms of Corynespora leaf spot disease from the suburbs of Nanchang were used as experimental materials.The methods such as tissue isolation，pathogenicity assays，observation of cultural characteristics and morphological traits，and molecular biological identification of the pathogen were used,the pathogen species of cucumber Corynespora leaf spot disease in the suburbs of Nanchang were studied，in order to provide a reference for rapid field diagnosis and scientific control of cucumber Corynespora leaf spot disease.The results showed that a total of 32 isolates with consistent cultural features and morphological characteristics were obtained from diseased cucumber leaves，which were all pathogenic to cucumber leaves.On PDA culture media，colonies of the tested strains were grayish white to grayish brown on the front side，and yellowish brown on the back side.The mycelia were dense and fluffy，with an average growth rate of 10 mm·d-1.The conidiophore were solitary，stout，erect or slightly bent，light brown with septa.The conidia were cylindrical，inverted rod-shaped，spindle or ‘Y’ shape，with multiple pseudo-septa，（22.8-137.8）μm×（4.1-12.9）μm in size，often solitary and occasionally concatenated.The cultural features and morphological characteristics of the strains were consistent with Corynesporacassiicolain the literature.Three representative strains had identical seqences of rDNA-ITS,ACT and [STBX]EF-1α[STBZ] genes，were 100% identity to the corresponding sequences of C.cassiicola in GenBank.In the phylogenetic tree based on the rDNA-ITS gene sequences，the representative strains was clustered into the same clade as the strains of C.cassiicola with 100% support.According to the morphological characteristics and analysis of the gene sequences，the pathogen of cucumber Corynespora leaf spot in suburbs of Nanchang was identified as C.cassiicola.

References:

［1］高思玉.设施黄瓜褐斑病的发病症状及综合防治措施［J］.上海蔬菜,2022(3):38-39.［2］樊斌,李明阳.中国黄瓜种植收益影响因素贡献率的比较分析［J］.北方园艺,2023(15):135-143.［3］WEI C T.Noteson Corynespora［J］.Myclilgical Papers，1950，30(34)：1-10.［4］GSSOW H T.ber eine neue Krankheit an Gurken in England (Corynespora mazei，Güssow gen.et spec.nov.)［J］.Zeitschrift für Pflanzenkrankheiten，1906，16(1)：10-13.［5］AGUIAR F M,VALLAD G E,TIMILSINA S,et al.Phylogenetic network analysis of South and North American Corynesporacassiicola isolates from tomato,cucumber,and novel hosts［J］.European Journal of Plant Pathology,2022,163(3):657-671.［6］戚佩坤,白金恺，朱桂香.吉林省栽培植物真菌病害志［M］.北京:科学出版社,1966.［7］高苇.我国黄瓜棒孢叶斑病的病原学及诊断技术的研究［D］.北京:中国农业科学院,2011.［8］刘鸣韬,徐瑞富,蒋学杰,等.黄瓜靶斑病研究初报［J］.河南农业科学,2003,32(8):33-35.［9］韩菊红，岳德成，姚博，等.平凉市设施蔬菜黄瓜棒孢叶斑病调查［J］.甘肃农业科技，2014(10)：33-35.［10］蓝国兵,谭耀华,何自福,等.黄瓜褐斑病(Corynespora cassiicola)在广东首次报道［J］.植物保护,2012,38(5):197-200.［11］DIXON L J,SCHLUB R L,PERNEZNY K,et al.Hostspecialization and phylogenetic diversity of Corynespora cassiicola［J］.Phytopathology,2009,99(9):1015-1027.［12］CARBONE I,KOHN L M.A method for designing primer sets for speciation studies in filamentous ascomycetes［J］.Mycologia,1999,91(3):553-556.［13］O′DONNELL K,KISTLER H C,CIGELNIK E,et al.Multiple evolutionary origins of the fungus causing Panama disease of banana:Concordant evidence from nuclear and mitochondrial gene genealogies［J］.Proceedings of the National Academy of Sciences of the United States of America,1998,95(5):2044-2049.［14］张贺,蒲金基,张欣,等.巴西橡胶树棒孢霉落叶病病原菌的生物学特性［J］.热带作物学报,2007,28(3):83-87.［15］裴月令,时涛,蔡吉苗,等.木薯棒孢霉叶斑病病原鉴定及其生物学特性测定［J］.热带作物学报,2011,32(4):728-733.［16］秦双林,王园秀,蒋军喜,等.江西奉新县猕猴桃叶斑病病原菌鉴定［J］.江西农业大学学报,2016,38(3):488-491.［17］贾敏,刘新涛,赵辉,等.芝麻棒孢叶斑病病原菌鉴定与致病力比较［J］.植物病理学报,2020,50(6):641-648.［18］谭海文,卢燕回,王雅,等.广西烤烟棒孢霉叶斑病病原分子鉴定及其生物学特性补充［J］.植物保护,2012,38(5):35-39,50.［19］薛彩英,武海燕,马庆周,等.河南省草莓棒孢叶斑病病原菌鉴定及其生物学特性［J］.植物保护学报,2023,50(2):402-412.［20］田守波,朱为民,朱龙英,等.番茄棒孢叶斑病病原鉴定及生物学特性研究［J］.植物保护,2020,46(3):157-162,166.［21］CHAI A L,DU G F,SHI Y X,et al.Corynespora spot of hot pepper caused by Corynespora cassiicola in China［J］.Canadian Journal of Plant Pathology,2014,36(3):407-411.［22］李明远,李兴红,张涛涛,等.辽宁发生茄子棒孢叶斑病［J］.植物保护,2001,27(6):48-49.［23］EDWARDS MOLINA J P,NAVARRO B L,ALLEN T W,et al.Soybean target spot caused by Corynespora cassiicola:A resurgent disease in the Americas［J］.Tropical Plant Pathology,2022,47(3):315-331.［24］刘志恒,李健冰,安心,等.辽宁省豇豆轮纹病病原菌鉴定及其生物学特性［J］.中国蔬菜,2013(18):88-95.

Memo

Memo:
-
Last Update: 2025-02-28