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Evaluation of Methods of Symptomatic Appearance on Different Apple Tissues Inoculated by Phytophthora cactorum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年21
Page:
23-29
Research Field:
Publishing date:

Info

Title:
Evaluation of Methods of Symptomatic Appearance on Different Apple Tissues Inoculated by Phytophthora cactorum
Author(s):
ZHANG Shengping1; ZHANG Nan2; MA Lei3; ZHAO Jiaqiang1; LYU Dezhi1; XU Guoliang1
(1.Shijiazhuang Institute of Fruit Trees，Hebei Academy of Agriculture and Forestry，Shijiazhuang，Hebei 050061；2.Hebei Provincial Pesticide Testing and Monitoring Station，Shijiazhuang，Hebei 050031；3.Quality and Safety of Agricultural Products Centre of Hebei Province，Shijiazhuang，Hebei 050011)
Keywords:
phytophthora rot; Phytophthora cactorum; apple; symptoms
PACS:
S 436.421.1
DOI:
10.11937/bfyy.20241780
Abstract:
Ripe fruits,young fruits and leaves treated with or without injury were used as the test materials.The suspension of Phytophthora cactorum was prepared by scraping the mold layer from the solid medium for inoculation test.The symptoms and manifestations of the suspension prepared by shaking culture of liquid medium after inoculation of three kinds of apple tissues treated with the same treatment were compared,in order to provide reference for improving the experimental efficiency of P.cactorum inoculation of apple.The results showed that the inoculated test of solid culture suspension of P.cactorum showed obvious symptoms in ripe fruits treated with injured treatment,but not in ripe fruits treated with injured treatment and young fruits treated with injured treatment.The bacterial suspension prepared by liquid shaking culture was used to carry out inoculation test.The injured and non-injured ripe fruits and young fruits were prone to develop symptoms,while the uninjured young fruits showed no obvious symptoms.The bacterial suspensions prepared by the two methods were easy to show symptoms in the leaves treated with or without injury,in order to improve the efficiency of apple phenotypic test,different apple tissues should choose the corresponding preparation method of Phytophthora cactorum suspension,and the inoculation object should choose apple leaves to show the most obvious disease.

References:

［1］van DER SCHEER H A T.The occurrence of crown rot caused by Phytophthora cactorum in the apple rootstock MM 104［J］.Netherlands Journal of Plant Pathology，1969，75(6)：369-370.［2］HARRIS D C.The Phytophthora diseases of apple［J］.Journal of Horticultural Science，1991，66(5)：513-544.［3］GROVE G G，BOAL R J.Influence of temperature and wetness duration on infection of immature apple and pear fruit by Phytophthora cactorum［J］.Phytopathology，1991，81(11)：1465-1471.［4］HORNER I J，WILCOX W F.Spatial distribution of Phytophthora cactorum in New York apple orchard soils［J］.Phytopathology，1996，86：1122-1132.［5］LATORRE B A，RIOJA M E，WILCOX W F.Phytophthora species as associated with crown and root rot of apple in Chile ［J］.Plant Disease，2001，85(6)：603-606.［6］FARZANEH M，SHARIFI-TEHRANI A，AHMADZADEH M，et al.Biocontrol of Phytophthora cactorum，the causal agent of root and crown rot on apple (Malus domestica) by formulated Pseudomonas fluorescens［J］.Communications in Agricultural and Applied Biological Sciences，2007，72(4)：891-900.［7］LIU F，LI B，LIAN S,et al.Effects of temperature and moisture on the infection and development of apple fruit rot caused by Phytophthora cactorum［J］.Plant Disease，2018，102(9)：1811-1819.［8］刘芳，李保华，刘家生，等.不同杀菌剂对苹果果实疫腐病的防治效果［J］.果树学报，2016，33(10)：1269-1276.［9］ELIZABETH B.The life history of Phytophthora cactorum (Leb.& Cohn) Schrot［J］.Transactions British Mycological Society，1943(26)：71-89.［10］ERWIN D C，RIBEIRO O K.Phytophthora diseases worldwide［M］.New York:American Phytopathological Society Press，1996.［11］李齐原.恶疫霉PCF/SCR效应分子编码基因的转录特征、克隆和功能分析［D］. 扬州：扬州大学，2015.［12］王晓敏，巩振辉，逯红栋，等.辣椒疫霉菌孢子诱导技术研究［J］.西北农业学报，2006，15(2)：59-62.［13］左豫虎，臧忠婧，刘惕若.影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件［J］.植物病理学报，2001，31(3)：241-245.［14］陈方新，齐永霞，高智谋，等.诱导疫霉菌产生游动孢子囊液体培养基的研制［J］.植物保护，2005，31(2)：34-37.［15］SEWELL G W F，WILSON J F，DAKWA J T.Seasonal variations in the activity in soil of Phytophthora cactorum，P.syringae and P.citricola in relation to collar rot disease of apple［J］.Annals of Applied Biology，1974，76(2)：179-186.［16］SNEH B，MCINTOSH D L.Studies on the behavior and survival of Phytophthora cactorum in soil［J］.Canadian Journal of Botany，1974，52(4)：795-802.［17］MCINTOSH D L.Effects of soil water suction，soil temperature，carbon and nitrogen amendments，and host rootlets on survival in soil of zoospores of Phytophthora cactorum［J］.Canadian Journal of Botany，1972，50(2)：269-272.［18］MADDEN L V，WILSON L L，YANG X，et al.Splash dispersal of Colletotrichum acutatum and Phytophthora cactorum by short-duration simulated rains［J］.Plant Pathology，1992，41(4)：427-436.［19］TYLTER B M.Molecular basis of recognition between Phytophthora pathogens and their hosts［J］.Annual Review of Phytopathology，2002，40：137-167.［20］CHEN X，WEN K，ZHOU X，et al.The devastating oomycete phytopathogen Phytophthora cactorum：Insights into its biology and molecular features［J］.Molecular Plant pathology，2023 (24)：1017-1032.

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Last Update: 2024-11-21