|Table of Contents|

Analysis of Simple Sequence Repeat (SSR) Loci and Marker Development in Eggplant (Solanum melongena L.) Pericarp Transcriptome

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年21
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Analysis of Simple Sequence Repeat (SSR) Loci and Marker Development in Eggplant (Solanum melongena L.) Pericarp Transcriptome
Author(s):
WANG Hong1; NIE Zhixing1; YANG Shuheng2; SHAO Zhiyong1; WANG Tonglin1; ZHENG Jirong1
(1.Vegetable Research Institute，Hangzhou Academy of Agricultural Sciences，Hangzhou，Zhejiang 310024；2.College of Plant Science and Technology，Huazhong Agricultural University，Wuhan，Hubei 430070)
Keywords:
eggplant (Solanum melongena L.); transcriptome of pericarp; SSR loci; marker development
PACS:
S 641.1
DOI:
10.11937/bfyy.20241712
Abstract:
Taking 36 568 transcripts from eggplant (Solanum melongena L.) pericarp samples as the test materials，SSR locus search was conducted using the MISA (MicroSAtellite) software.The distribution frequency and characteristics of SSR loci were studied，and primers for the retrieved SSR loci were designed using Primer 3.0 software，while verifying the effectiveness of the primers,in order to provide reference for germplasm identification，phylogenetic analysis，molecular marker assisted breeding，and genetic map construction of eggplants.The results showed that a total of 9 775 SSR loci were detected，distributed on 6 289 transcripts.The frequency of the SSR loci was 26.73%，with an average distance of 5.49 kb.SSR loci characteristics analysis revealed that mononucleotide was the dominant repeat motif type，accounting for 47.67% of the total number of SSRs.The A/T motif was the most numerous repeat motif type，accounting for 46.13% of the total.97.91% of the repeated motifs had a repetition frequency between 5-25，and 92.21% of the SSR sequence length was mainly distributed between 10-30 bp.After primer design for all SSR loci，a total of 6 619 pairs of EST-SSR primers were obtained.27 pairs of EST-SSR primers were randomly selected for PCR amplification in 12 eggplant inbred lines.The validity of the primer was 92.59%，and the polymorphism rate was 33.33%.

References:

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Last Update: 2024-11-21