|Table of Contents|

Diversity of Rhizosphere Microbes and Screening of Growth-promotion Microorganisms From Xinyang Maojian Tea Plant

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年21
Page:
61-69
Research Field:
Publishing date:

Info

Title:
Diversity of Rhizosphere Microbes and Screening of Growth-promotion Microorganisms From Xinyang Maojian Tea Plant
Author(s):
ZHU Qingsong1; GU Qingyi2; QIAO Xinrong2; ZHU Nailiang2; YE Run2
(1.Horticultural College，Xinyang Agriculture and Forestry University，Xinyang,Henan 464000；2.School of Pharmacy，Xinyang Agriculture and Forestry University，Xinyang,Henan 464000)
Keywords:
Xinyang Maojian tea plant; rhizosphere microbes; dominant microbial community; growth-promoting microorganisms
PACS:
S 571.1；S 154.3
DOI:
10.11937/bfyy.20240927
Abstract:
Taking the rhizosphere soil of Xinyang Maojian tea plant as the test material,high-throughput amplicon sequencing method was used to analyze the diversity of rhizosphere bacteria and fungi,and the growth promoting characteristics of the isolated rhizosphere microorganisms were analyzed by selection medium,colorimetry,antagonism,in order to provide reference for the development and research of rhizosphere microbial fertilizers for tea plants in the future.The results showed that the isolated bacteria were 35 phyla,98 classes,222 orders,378 families,707 genera,and a total of 1 425 species,and fungi were belong to 7 phyla,27 classes,70 orders,147 families,242 genera,and a total of 332 species.The dominant bacterial communities at phylum level were mainly Proteobacteria,Acidobacteriota and Actinomycetota,with relative abundance of 32.42%,20.84% and 12.28% respectively,and the dominant genera were Bradyrhizobium (6.27%),Acidobacterium (4.48%) and WPS-2 (3.45%).Then,the dominant fungal communities at phylum level were mainly Ascomycota,Basidiomycota and Fungi unclassified,with relative abundance of 45.97%,28.21% and 23.64% respectively,and the dominant genera were mainly unknown fungal genera (23.64%) and unknown genera of ascomycetes (21.34%).A bacterial strain B25 was screened and identified as Burkholderia cepacia with the ability of dissolving phosphorus and producing siderophores.The F2 strain,which had the characteristics of dissolving phosphorus,producing siderophores,and antagonizing pathogenic fungi,had been identified as Talaromyces sp..The F8 strain with characteristics of secreting IAA,producing siderophores,and antagonizing pathogenic fungi had been identified as Alternaria alternata.The F9 strain,which had the characteristics of secreting IAA and antagonizing pathogenic fungi,had been identified as Gongronella koreana.The rhizosphere microorganisms of Xinyang Maojian tea plant had local specificity at the genus and species level,and four growth- promoting microorganisms with multiple efficacy were isolated and screened.

References:

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Last Update: 2024-11-21