|Table of Contents|

Study on the Inhibitory Effect and Mechanism of Composite Probiotics on Leucosis of Cordyceps militaris

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年21
Page:
83-91
Research Field:
Publishing date:

Info

Title:
Study on the Inhibitory Effect and Mechanism of Composite Probiotics on Leucosis of Cordyceps militaris
Author(s):
ZHANG Jinying1; HE Xi1; LI Bingxin1; LIU Yefei2; 3; 4; WANG Ze1; 5; XU Fangxu2; 4; 5
(1.College of Life Science，Shenyang Normal University，Shenyang，Liaoning 110034;2.Experimental Teaching Center，Shenyang Normal University，Shenyang，Liaoning 110034;3.Shenyang Functional Cordyceps Militaris Industry Technology Research Institute，Shenyang，Liaoning 110034;4.Cordyceps Militaris Germplasm Bank of Liaoning Province，Shenyang,Liaoning 110034;5.Key Laboratory of Functional Cordyceps Militaris in Liaoning Province，Shenyang,Liaoning 110034)
Keywords:
Cordyceps militaris; composite probiotics; leucosis; transcriptome; differentially expressed genes
PACS:
S 853.75
DOI:
10.11937/bfyy.20240545
Abstract:
Cordyceps militaris with compound probiotics added and fresh Cordyceps militaris in control group were used as test materials，transcriptome sequencing was analyzed by high-throughput sequencing technology，and functional annotation and pathway analysis of differential genes were performed by bioinformatics methods.The effect of adding complex probiotics to induce resistance to white disease on gene expression changes of Cordyceps militaris during cultivation was studied,in order to provide reference for adding compound probiotics during the cultivation process to improve the disease resistance of Cordyceps militaris.The results showed that Cordyceps militaris with the addition of composite probiotics had a significant disease resistance effect，with a 71.1% decrease in disease index and a total of 801 differentially expressed genes.Enriched into 20 metabolic pathways.Differential genes were mainly involved in ethylene signal transduction，regulating the synthesis of plant antitoxins and other secondary metabolites related to cell wall thickening，as well as the metabolism of lysine and phenylalanine.The addition of composite probiotics could effectively activate the MAPK signaling pathway plant pathogen interaction and lysine degradation pathway，thereby improving the disease resistance of Cordyceps militaris during its growth process.

References:

［1］林培捷,陈柏雄,陈乐涛,等.高产虫草素的蛹虫草新品种选育［J］.食品与生物技术学报,2022,41(3):59-65.［2］PROMMABAN A,SRIYAB S,MARSUP P,et al.Comparison of chemical profiles,antioxidation,inhibition of skin extracellular matrix degradation,and anti-tyrosinase activity between Mycelium and fruiting body of Cordyceps militaris and Isaria tenuipes［J］.Pharmaceutical Biology,2022,60(1):225-234.［3］李梦雪,王升厚,柳叶飞,等.功能性和普通蛹虫草的差异代谢物对比分析［J］.食品研究与开发,2022,43(3):34-41.［4］KUNHORM P,CHUEAPHROMSRI P,CHAICHAROENAUDOMRUNG N,et al.Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification［J］.Biotechnology Letters,2022,44(4):581-593.［5］WANG Y,YANG X,CHEN P,et al.Homologous overexpression of genes in Cordyceps militaris improves the production of polysaccharides［J］.Food Research International,2021,147:110452.［6］DO T,NGUYEN H C.Extraction of cordycepic acid from the fruiting body of Cordyceps militaris (L.)［J］.BioTechnologia,2019,100(3):219-226.［7］YU X Y,ZOU Y,ZHENG Q W,et al.Physicochemical,functional and structural properties of the major protein fractions extracted from Cordyceps militaris fruit body［J］.Food Research International,2021,142:110211.［8］JIN Y Q,LI B.Cultivation of eri-silkworm Cordyceps militaris and determination of its amino acid composition［J］.Advanced Materials Research,2013,796:21-24.［9］DANG H N,WANG C L,LAY H L.Effect of nutrition,vitamin,grains,and temperature on the Mycelium growth and antioxidant capacity of Cordyceps militaris (strains AG-1 and PSJ-1)［J］.Journal of Radiation Research and Applied Sciences,2018,11(2):130-138.［10］SHI K Y,YANG G,HE L,et al.Purification,characterization,antioxidant,and antitumor activity of polysaccharides isolated from silkworm Cordyceps［J］.Journal of Food Biochemistry,2020,44(11):e13482.［11］于悦.蛹虫草胞外多糖的分离纯化及其免疫活性研究［D］.长春:吉林农业大学,2021.［12］郐滨.北虫草菌素制备及其抗炎、免疫调节活性研究［D］.哈尔滨:东北林业大学,2021.［13］刘晴,万佳欣,张雨晨,等.蛹虫草病原真菌虫草生齿梗孢Calcarisporium cordycipiticola的生物学特性研究［J］.菌物学报,2018,37(8):1054-1062.［14］孟小林,祁巧芬,陈英,等.日粮添加复合益生菌对肉鸡生长性能、免疫功能及肠道健康的影响［J］.饲料研究,2022,45(15):52-56.［15］LIU H X,KANG X,YANG X D,et al.Compound probiotic ameliorates acute alcoholic liver disease in mice by modulating gut microbiota and maintaining intestinal barrier［J］.Probiotics and Antimicrobial Proteins,2023,15(1):185-201.［16］门先会.益生菌介导肠道菌群及其代谢产物调控虹鳟生长与免疫的机制研究［D］.青岛:青岛农业大学,2022.［17］崔芙蓉,王升厚,李梦雪,等.生长调节剂GN-01对蛹虫草寄生性病原真菌的诱导抗病效果［J］.南方农业,2022,16(17):94-97.［18］陈珣,肇莹,肖军.不同蛹虫草品种对白毛病抗病性的初步评价［J］.北方园艺,2021(21):117-122.［19］刘晴,董彩虹.蛹虫草病原菌虫草生齿梗孢遗传转化体系构建及互作研究［C］//多彩菌物 美丽中国:中国菌物学会2019年学术年会论文集.西安,2019:119.［20］孟国良,王徐萍,刘梦潜,等.蛹虫草高效〖STBX〗CRISPR/Cas9〖STBZ〗基因编辑技术［C］.贵阳：中国菌物学会2023年学术年会,2023.［21］陈康.平菇黄斑病病原物种类多样性与品种抗病性初步研究［D］.武汉:华中农业大学,2018.［22］张钰,唐明.丛枝菌根真菌对青杨抗溃疡病生物量和抗病酶活性的影响［J］.菌物学报,2021,40(5):1110-1122.［23］刘元涛.蛹虫草富集铬离子的生理生化机制研究［D］.烟台:鲁东大学,2022.［24］张菊华,王伟,林树花,等.丁香、五味子及壳聚糖复合保鲜剂对蓝莓贮藏品质及抗病酶活的影响［J］.中国食品学报,2017,17(12):139-148.［25］何静,张小彦,陈伟,等.马铃薯糖苷生物碱处理后枸杞鲜果的转录组学分析［J］.植物保护学报,2021,48(3):662-668.［26］安莉,汪红,李萌,等.基于转录组的氯吡脲对草莓果实成熟的调控机制研究［J］.食品安全质量检测学报,2021,12(14):5636-5643.［27］SUN X H,YU G,LI J T,et al.AcERF2,an ethylene-responsive factor of Atriplex canescens,positively modulates osmotic and disease resistance in Arabidopsis thaliana［J］.Plant Science,2018,274:32-43.［28］李娜,张文玉,孙纲,等.植物SnRK2基因家族的研究进展［J］.中国农学通报,2023,39(30):108-113.［29］徐良伟,吴小祝,贾明良,等.MAPK级联及其在植物抗病防卫反应中的研究进展［J］.激光生物学报,2019,28(6):488-495.［30］孙琛琛.血脂异常肝肾阴虚、气滞血瘀、阴虚阳亢三证的代谢组学研究［D］.北京:北京中医药大学,2017.［31］赵利,钞建宾,郭捷,等.基于代谢组学技术的植物抗病相关代谢物研究进展［J］.西北植物学报,2021,41(6):1071-1078.［32］孙翠.酵母细胞壁对梨和番茄果实采后病原真菌抗性的诱导作用及相关机理研究［D］.杭州:浙江大学,2019.［33］王俊青，张彦辉，易婉莹,等.基于转录组测序红秋葵果实转色期花青素生物合成相关基因研究［J］.北方园艺,2022(14):9-16.［34］BI G,ZHOU Z,WANG W,et al.Receptor-like cytoplasmic kinases directly link diverse pattern recognition receptors to the activation of mitogen-activated protein kinase cascades in Arabidopsis［J］.The Plant Cell,2018,30(7):1543-1561.［35］黄耘,张爱丽,苏蕊,等.KMT2A通过lncRNA-HOTAIR激活Akt/mTOR信号通路影响AML细胞的生物学行为［J］.中国实验血液学杂志,2022,30(3):732-736.

Memo

Memo:
-
Last Update: 2024-11-21