|Table of Contents|

Effects of 137Cs-γ Ray Radiation on Callus Growth and Differentiation of Clematis courtoisii

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年17
Page:
103-108
Research Field:
Publishing date:

Info

Title:
Effects of 137Cs-γ Ray Radiation on Callus Growth and Differentiation of Clematis courtoisii
Author(s):
HAN Xin1; LIU Chaogang2; GAO Lulu1; ZHANG Mengchao2; WANG Shu′an1; LI Linfang2
(1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing,Jiangsu 210014;2.Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021)
Keywords:
137Cs-γ radiation; Clematis courtoisii; callus differentiation
PACS:
S 853.75
DOI:
10.11937/bfyy.20240381
Abstract:
Taking the embryonic roots of Clematis courtoisii as the experimental material,embryogenic callus was induced.Hormone ratio and radiometric analysis methods were used to study the effects of hormone ratio and radiometric analysis on callus differentiation,in order to provide reference for the research on tissue culture and mutation germplasm innovation of Clematis courtoisii.The results showed that 6-BA 2 mg· L-1+NAA 0.05 mg· L-1 was the optimal medium for inducing callus differentiation.By using different doses of 137Cs-γ rays (0,30,60,90,120,150,180 Gy) for radiation treatment,the radiation dose of 60 Gy had the least impact on callus differentiation rate,and 120 Gy was the semi lethal dose of Clematis courtoisii.The appropriate dose range for 137Cs-γ radiation in the callus tissue of Clematis courtoisii was 60-90 Gy.

References:

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Last Update: 2024-09-24