|Table of Contents|

Optimization of Aqueous Two-phase Extraction of Polyphenols From Cirsium setosum and Investigation Into Their Biological Activity

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年11
Page:
88-96
Research Field:
Publishing date:

Info

Title:
Optimization of Aqueous Two-phase Extraction of Polyphenols From Cirsium setosum and Investigation Into Their Biological Activity
Author(s):
CAI Jing1; ZHANG Jingyi1; YE Run1; DUAN Baoqing1; HUANG Yaqin1; LIU Xinhao2
(1.School of Pharmacy，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000；2.Central Laboratory，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000)
Keywords:
Cirsium setosum; polyphenols; aqueous two-phase extraction; α-amylase; α-glucosidase; nitrite
PACS:
R 284.2
DOI:
10.11937/bfyy.20234489
Abstract:
Aqueous two-phase extraction (ATPE) was adopted to extract polyphenols from Xinyang Cirsium setosum.The extraction process of polyphenols was studied，while also investigated its inhibitory effects on starch-digesting enzyme activity in vitro and nitrite remove ability，in order to provide reference for the development and utilization of polyphenols as hypoglycemic drugs or food additives.The results showed that the optimal extraction conditions were as follows,(NH4)2SO4 concentration was 23%，ethanol concentration was 26%，extraction time was 60 minutes，liquid-solid ratio was 30∶1 mL·g-1，extraction temperature was 81 ℃.Under these conditions,the yield of polyphenols was (9.36±0.51)mg·g-1，and the IC50 values for inhibition of α-amylase and α-glucosidase were 0.014 mg·mL-1 and 0.061 mg·mL-1，respectively，which were significantly superior to acarbose.Additionally，the nitrite clearance rate was as high as 82.05%.It showed excellent hypoglycemic activity and remarkable nitrite removal ability.

References:

［1］WANG B,LV D,HUANG P,et al.Optimization,evaluation and identification of flavonoids in Cirsium setosum (Willd.) MB by using response surface methodology［J］.Journal of Food Measurement and Characterization,2019,13(2):1175-1184.［2］WANG H C,BAO Y R,WANG S,et al.Simultaneous determination of eight bioactive components of Cirsium setosum flavonoids in rat plasma using triple quadrupole LC/MS and its application to a pharmacokinetic study［J］.Biomedical Chromatography,2019,33(11):e4632.［3］CHEN Y，DING A W，YANG X H，et al.Review of Cirsium setosum about chemical constituents，pharmacological action and clinical application［J］.Study Journal of Traditional Chinese Medicine，2005，23：614-615.［4］LUO W,WU B,TANG L J,et al.Recent research progress of Cirsium medicinal plants in China［J］.Journal of Ethnopharmacology,2021,280:114475.［5］杨炳友,杨春丽,刘艳,等.小蓟的研究进展［J］.中草药,2017,48(23):5039-5048.［6］GAO Y,WANG S,DANG S,et al.Optimized ultrasound-assisted extraction of total polyphenols from Empetrum nigrum and its bioactivities［J］.Journal of Chromatography B,2021,1173:122699.［7］FENG J,KONG F.Enzyme inhibitory activities of phenolic compounds in pecan and the effect on starch digestion［J］.International Journal of Biological Macromolecules,2022,220:117-123.［8］武洪敏,高青青,陈庚,等.双水相法萃取白及多糖及其对亚硝酸盐的清除作用［J］.北方园艺,2021(3):114-121.［9］ALU′DATT M H,RABABAH T,ALHAMAD M N,et al.Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems:Content,and antioxidant,anti-diabetic and anti-hypertensive properties［J］.Food & Function,2017,8(9):3187-3197.［10］马巧宁.冻绿叶多酚的提取纯化及抗氧化性能研究［D］.太原:山西大学,2021.［11］〖AKI·〗SIMEN E M,BERKTAS S,AM M,et al.Optimization of ultrasound-assisted aqueous two-phase system (ATPS) of phenolics from apple pulp and peel［J］.Preparative Biochemistry & Biotechnology,2023,53(2):207-214.［12］李品月,张威明,陶俊,等.应用响应面法优化芍药花瓣多酚超声提取工艺［J］.北方园艺,2022(23):112-119.［13］WU Y,LIU H,LI Z,et al.Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro［J］.Preparative Biochemistry & Biotechnology,2022,52(1):89-98.［14］郑晶晶,张晓华,刘秀平,等.蜂蜜中多酚类化合物的超声辅助双水相提取工艺研究［J］.食品研究与开发,2022,43(17):63-70.［15］赵丽平,刘琦,蔡静,等.刺槐花多糖蛋白质脱除工艺优化及生物活性测试［J］.中国食品添加剂,2022,33(9):63-68.［16］WANG Z,PENG S,PENG M,et al.Adsorption and desorption characteristics of polyphenols from Eucommia ulmoides Oliv.leaves with macroporous resin and its inhibitory effect on α-amylase and α-glucosidase［J］.Annals of Translational Medicine,2020,8(16):1004.［17］李佩艳,韩四海,罗登林,等.牡丹叶黄酮的酶法提取条件优化及其对亚硝酸盐的清除作用［J］.食品科学,2016,37(6):77-81.［18］JI Y W,RAO G W,XIE G F.Ultrasound-assisted aqueous two-phase extraction of total flavonoids from Tremella fuciformis and antioxidant activity of extracted flavonoids［J］.Preparative Biochemistry & Biotechnology,2022,52(9):1060-1068.［19］周飙,胡斌,柳鑫,等.超声辅助双水相提取绞股蓝皂苷工艺及其降糖活性分析［J］.食品工业科技,2023,44(20):197-205.［20］ZHOU H,FENG X F,YAN Y,et al.Optimization of an ultrasonic-assisted aqueous two-phase extraction method for four flavonoids from Lysionotus pauciflorus［J］.Preparative Biochemistry & Biotechnology,2022,52(7):770-782.［21］杨子敬,饶桂维,王磊.超声波辅助双水相提取枇杷花总黄酮工艺优化及其抗氧化性［J］.食品工业科技,2021,42(19):218-225.［22］JI Y W,RAO G W,XIE G F.Ultrasound-assisted aqueous two-phase extraction of total flavonoids from Tremella fuciformis and antioxidant activity of extracted flavonoids［J］.Preparative Biochemistry & Biotechnology,2022,52(9):1060-1068.［23］竺静,陈晶晶,姚佳宁.不同提取方式下小蓟、小蓟炭提取物抗炎、凝血活性的比较［J］.浙江海洋大学学报(自然科学版),2019,38(3):254-258.［24］葛水莲,陈建中,张淑娟,等.小蓟不同提取物离体抑菌活性研究［J］.北方园艺,2014(10):109-111.

Memo

Memo:
-
Last Update: 2024-06-27